Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (6): 652-655.

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Proteomic Analysis of Differential Protein Expression in 293T Cells after Overexpression of Enterovirus 71 3C

Xiao-bo LEI1,Zhen-min SUN2,Xin-lei LIU2,Li-li REN2,Jian-wei WANG3   

  1. 1. Institute of Pathogen Biology, CAMS&PUMC
    3. Institute of Pathogen Biology, CAMS& PUMC
  • Received:2011-03-09 Revised:2011-04-11 Online:2011-06-05 Published:2011-06-06
  • Contact: Jian-wei WANG

Abstract: Objective To explore the effects of 3C protease on host cell proteins by proteomics techniques. Methods 293T cells were transfected with plasmid encoding EV71 3C, cell lysates were analyzed by using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS) analysis. Down-regulated protein was confirmed by Western blot analysis. Results The expression of EV71 3C in 293T cells is confirmed. A total of 5 altered proteins were identified after 3C overexpression. Among them, expression of 4 proteins increased, while expression of 1 protein decreased. Down-regulated protein HSPA4 was confirmed by Western blot. HSPA4 expression was determined by performing densitometric analyses in HeLa cells. The results show that HSPA4 expression was 46.8%±10.1% (P<0.05) compared to normal cells. Conclusions Overexpression of EV71 3C regulates the protein expression in 293T cells.

Key words: Enterovirus 71, 3C, proteomics differences