Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (6): 642-646.

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MicroRNA-144 Regulates Erythroid Differentiation of K562 Cells

Xiao-yan WANG1,Chong-liang JIANG1,Yong ZHU2,Fang WANG2,Jia YU3,Tao FENG1   

  1. 1. Molecular Medicine&Cancer Research Center, Institute of Basic Medical Sciences, Chongqing Medical University
    3. Institute of Basic Medical Sciences, CAMS&PUMC
  • Received:2011-02-28 Revised:2011-04-02 Online:2011-06-05 Published:2011-06-06
  • Contact: Tao FENG

Abstract: Objective: We aimed to study the influence of miR-144 on human erythroid differentiation via targeting the retinoblastoma protein ( RB). Methods: K562 cells were treated with hemin to differentiate into mature red cells. Real-time PCR analysis was used to detect the changes of miR-144 expression during erythroid differentiation. K562 cells were transfected with oligonucleotides (mimic-144) and the influence of erythropoiesis was measured. The target gene of miR-144 was identified by using bioinformatics analysis combined with Dual-luciferase reporter and Western blot analysis. Results: MiR-144 was significantly increased during hemin-induced K562 erythroid differentiation(P<0.05). Over-expression of miR-144 in K562 cells promoted the stimulation of γ-globin and CD235a. Moreover, RB was identified as a direct target of miR-144. Expression of RB increased slightly and then reduced obviously in erythroid differentiation . Grey mean of RB/GAPDH minimum point: 12h 0.092±0.007(P<0.05 compared with 0h). Conclusion: MiR-144 played a positive role in erythroid differentiation of K562 cells via targeting RB.

Key words: microRNA-144, erythroid differentiation, RB

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