Abstract: 【Absrtact】：Objective：To establish rat model of cold acclimation by different cold exposure，and to form foundation on studying the molecule mechanisms of cold acclimation. Methods:60Sprague-Dawley rats were randomly divided into 3groups with 20 in each, control group ,4℃cold exposure 4h/d group,0℃cold exposure 10h/d group.The plasma levels of monoamine neurotransmitters were analyzed by HPLC-ECD techniques;the content of plasma corticosterone was evaluated by radioactivity immunoassay；myeloperoxidase(MPO) activity of the myocardium were measured by colorimetric method.Results:In different cold stress,plasma levels of monoamine neurotransmitters and corticosterone significantly different（P＜0.05）.Compared with the cold exposure group，the plasma LDH,CK-MB and myocardium MPO activities in cold acclimation rats after cold exposure were significantly decreased（P＜0.05）.Conclusion: cold acclimation rat model is established，and cold acclimation can attenuate the myocardial damage caused by cold exposure.

Key words: cold acclimation, cold exposure, Hormone, Myocardial damage, cold acclimation, cold exposure, Hormone, Myocardial damage