Abstract: Objective To explore the role of connective tissue growth factor（CTGF）in the mechanism of liver fibrosis. Methods Liver fibrosis models of rats were made by subcutaneously injecting with CCl4. On the other hand, 32 human liver cirrhosis and 12 normal liver tissues were collected too. Immunohistochemical staining was used to detect the expression of CTGF in these tissues. Results The expression of CTGF in rats after injection with CCl4 were found chiefly in hepatic stellate cells(HSC) and hepatocytes and all significantly higher than those in normal rats(P<0.01,P<0.05)..Moreover, it present with progressive tendency for the expression of CTGF in rats respectively at 1st,4th and 8th week after injection with CCl4(P<0.01,P<0.05). The same tendency was found in human normal and fibrotic liver tissues(P<0.01) too. Conclusions The overexpression of CTGF may accelerate the procedure of liver fibrosis via enhancing HSC proliferation and the ECM increasing, and play an important role in liver fibrogenesis. , and their liver tissues were used for examining CTGF by immunohistochemistry stain.