Basic & Clinical Medicine ›› 2007, Vol. 27 ›› Issue (1): 25-30.
• 研究论文 • Previous Articles Next Articles
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Abstract: Abstract : Objective: The objective was to look for cocaine-and amphetamine-regulated transcript peptide(CART) receptors or proteins interacted to CART. Method :A cDNA library from rat brain was constructed. Then the BacterioMatch two-hybrid system was used to screening for proteins interacted with CART by using CART41-102 as bait. Result:The cDNA library and bait pBT- CART41-102 were constructed successfully. The library harvested 3.37×106 individuals, and the reconstruction rate was 98.5%. 5.01x106 cotransformed clones were screened, 93 clones were conformtively identified to interacting with CART and were DNA sequenced. By bioinformation analysis, 6 known proteins interacted to CART were identified. Other unknown interaction proteins that had short exactly similiar motif to 22 membraneproteins or receptors were also identified. Conclusion: CART might interact to proteins about energy balance, cell proliferation and enzymes related to protein processing and degrading .
Key words: cDNA library, CART, bacterial two-hybrid system, protein-protein interaction
. Screening of proteins interacting with neuropeptide CART[J]. Basic & Clinical Medicine, 2007, 27(1): 25-30.
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https://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2007/V27/I1/25