Basic & Clinical Medicine ›› 2023, Vol. 43 ›› Issue (5): 809-816.doi: 10.16352/j.issn.1001-6325.2023.05.0809

• Technology and Methodology • Previous Articles     Next Articles

Comparative investigation for auto-fluorescence sample detection by evaluating conventional and spectral flow cytometers performance

CHEN Jiahuan1, LI Dandan2, SUN Yan1, WANG Yilei3, WANG Duo3, KONG Jie1, LIU Xiaoling1*   

  1. 1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC, Beijing 100005;
    2. Department of Laboratory Medicine, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730;
    3. Changzhi Medical College, Changzhi 046000, China
  • Received:2023-01-17 Revised:2023-03-21 Online:2023-05-05 Published:2023-04-26
  • Contact: *

Abstract: Objective To compare the performance of conventional and spectral flow cytometers in detecting auto-fluorescence samples. Methods Single cell samples with auto-fluorescence were prepared and stained by annexin V(AV)binding and propidium iodure (PI) to detect apoptosis. The same auto-fluorescence sample was run on conventional and spectral flow cytometers side by side. Results By avoid using FITC in conventional cytometers, the better result was gotten in detecting auto-fluorescence samples. Full spectrum cytometry presented better resolution even there was spectral overlap. It was easy to discriminate the signals derived from auto-fluorescence and FITC. The protocol of annexinV binding and PI staining to analyze apoptosis was optimized. Conclusions Spectral flow cytometry shows relatively excellent performance under certain conditions in detecting of apoptosis derived from auto-fluorescence samples.

Key words: auto-fluorescence, flow cytometry, spectrum, fluorescein isothiocyanate(FITC)

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