Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (11): 1690-1696.doi: 10.16352/j.issn.1001-6325.2022.11.1690

• Original Articles • Previous Articles     Next Articles

miR-34c-5p knockdown inhibits hypoxia induced cell apoptosis of bone marrow derived mesenchymal stem cells in rats

DONG Yang, ZHANG Fen, LI Xing-xing, WU Jie, XU Zhong-cheng*   

  1. The Second Department of Cardiology, Jinhua People's Hospital, Jinhua 321099,China
  • Received:2021-05-24 Revised:2022-02-18 Online:2022-11-05 Published:2022-11-01
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Abstract: Objective To explore the effect of miR-34c-5p knockdown on improving the survival rate of bone marrow derived mesenchymal stem cells (BM-MSCs). Methods The BM-MSCs were divided into normoxia group, hypoxia group, normoxia inhibitor NC group, hypoxia inhibitor NC group, normoxia microRNA(miR) inhibitor group and hypoxia miR inhibitor group. The expression level of miR-34c-5p, IGF, HGF, bFGF and VEGF was detected by real-time quantitative PCR (RT-qPCR) and ELISA. Cell apoptosis was detected by flow cytometry and TUNEL. The protein expression levels of cleaved caspase-3, -9, Bcl-2 and Bax were detected by Western blot. ATP/ADP was detected by biochemical kits, and glucose uptake was detected by flow cytometry. Results Compared with hypoxic inhibitor NC group, miR-34c-5p knockdown significantly inhibited BM-MSCs apoptosis(P<0.01), reduced protein expression of cleaved caspase-3, -9 and Bax and increased protein expression by Bcl-2 (P<0.01). The expression of IGF, HGF, bFGF and VEGF (P<0.05, P<0.01) were significantly increased; ATP/ADP rate and cell capacity of glucose transporting also increased (P<0.05, P<0.01). Conclusions miR-34c-5p knockdown may antagonize hypoxia induced cell apoptosis of BM-MSCs in rat.

Key words: miR-34c-5p knockdown, bone marrow derived mesenchymal stem cells, hypoxia, cell apoptosis

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