Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (10): 1561-1566.doi: 10.16352/j.issn.1001-6325.2022.10.1561

• Original Articles • Previous Articles     Next Articles

Expression of EphB2 receptor at different stages of functional recovery in cerebral ischemia rats

ZHANG Jie-ting, LI Hai-xi, WANG Ting-ting, YUN Lu, YANG Hai*   

  1. Department of Pharmacy, Qingdao Central Hospital, Qingdao 266042, China
  • Received:2021-09-14 Revised:2022-02-18 Online:2022-10-05 Published:2022-09-23
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Abstract: Objective To investigate the expression of erythropoietin-producing hepatocyte B2 (EphB2) receptor at different phases of functional recovery after middle cerebral artery occlusion(MCAO) in rats. Methods Sixty-four rats were divided into four groups:sham group and 2, 14 and 28 days after MCAO. The neurological and motor function was evaluated with a modified mNSS score at 2 d, 14 d or 28 d after surgery. The mRNA and protein in the hippocampus and cortex were measured by q-PCR and Western blot, respectively. Results Rats showed the highest mNSS score at 2 d after MCAO as compared with sham group (P<0.001) and the score decreased with time.The protein level of EphB2 receptor in the hippocampus increased significantly at 2 d after MCAO (P<0.05)compared with sham group, and then gradually decreased at 14 and 28 d after MCAO. The expression of EphB2 receptor showed correlation with mNSS scores. However, the expression of EphB2 receptors in the cortex and the EphB2 mRNA level in the hippocampus did not change significantly after MCAO. Conclusions The expression of EphB2 receptor in the hippocampus is up-regulated at early phase of MCAO and then decreased, which is closely correlated with functional score in rats.

Key words: EphB2 receptor, cerebral ischemia, functional recovery, different stages

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