Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (10): 1509-1513.doi: 10.16352/j.issn.1001-6325.2022.10.1509

• Original Articles • Previous Articles     Next Articles

Balanophora polysaccharide protects against neuron damage in rat model of spinal cord injury

ZHAO Fang-yu1,2, QIN Xiao-li2, HE Yi-duo1, WANG Feng-jie1,2, TANG Xian-e1,2, PENG Xin-xin3, CHEN Xian-bing1,2*   

  1. 1. Department of Pathology,Minda Hospital of Hubei Minzu University, Enshi 445000;
    2. Department of Medicine, Hubei Minzu University, Enshi 445000;
    3. College of Medicine, Hubei Enshi College,Enshi 445000,China
  • Received:2021-07-28 Revised:2022-01-25 Online:2022-10-05 Published:2022-09-23
  • Contact: *

Abstract: Objective To investigate the effect of balanophora polysaccharide (BPS) on neuro-autophagy and its therapeutic effect on spinal cord injury. Methods The rats were randomly divided into sham operation group(sham, n=10),model group(SCI, n=15,which were established according to the Allen's weight-striking method), and the BPS treatment group(BPS, n=45),which were given intra-gastrically with low, medium and high dose of BPS solution for 14 days). The BBB scores were measured to evaluate motor function. Hematoxylin-Eosin and Nissl staining was used to observe the morphological changes. The expression of autophagy markers of Beclin 1, LC3-Ⅱ and p62 was detected by Western blot. Results Compared with the sham operation group,the curve of BBB score-time in SCI group was significantly lower and the injury was severer(P<0.05). In SCI group, the dorsal white matter and central gray matter of spinal cord were seriously injured with obvious cavity. Further observation showed that the number of neurons in SCI group was significantly decreased; the structure of cell body was incomplete. The result of Western bolt showed that the relative change of LC3 Ⅱ /Ⅰ and p62 was up-regulated on day 1,7 and 14, the highest expression of both protein was found on day 7(P<0.05). At the same time,Beclin 1 was decreased after injury with lowest expression on day 7(P<0.05). The BBB scores of BPS group were increased with a dose-dependent manner as compared with SCI group. The cavity of dorsal white matter and central gray matter shrinked. The number of neurons was increased with intact morphology. The expression of Beclin 1 and LC3 Ⅱ/Ⅰ was increased significantly in BPS group, while p62 decreased as compared to SCI group(P<0.05). Conclusions BPS has a well-demonstrated neuron-protective effect and the underlying mechanism might be related to the protection of injured nerve cells.

Key words: Balanophora polysaccharide, spinal cord injury, autophagy

CLC Number: