Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (8): 1200-1205.doi: 10.16352/j.issn.1001-6325.2022.08.1200

• Original Articles • Previous Articles     Next Articles

Curcumol inhibits proliferation and promotes apoptosis of human osteosarcoma cell lines

JIANG Ning1, GUO Jun2*, LIU Cheng1, ZHOU Ju1   

  1. 1. Department of Orthopedics, the Fifth Medical Center, Chinese People’ s Liberation Army General Hospital(the former 307th hospital of PLA), Beijing 100007;
    2. Department of Orthopedics, the First Affiliated Hospital of Tsinghua University, Beijing 100016, China
  • Received:2022-02-22 Revised:2022-05-06 Online:2022-08-05 Published:2022-08-02
  • Contact: *gzd713@163.com

Abstract: Objective To explore the effects and mechanism of curcumol on in vitro proliferation and apoptosis of human osteosarcoma cell lines MG-63 and U2OS. Methods MG-63 cells and U2OS cells were divided into control group and 20, 40, 80 and 160 μmol/L curcumol groups. MTT assay was used to detect the proliferation levels of MG-63 and U2OS cells, and Transwell chamber assay was used for cell migration and invasion and flow cytometry was used for cell apoptosis. RT-qPCR and Western blot were applied to detect the mRNA and protein expressions of apoptosis-related proteins Bax, Bcl2, caspase and β-catenin. Results Curcumol inhibited the proliferation of MG-63 and U2OS cells in a concentration-dependent manner, and the IC50 values were 128.03 μmol/L and 117.95 μmol/L respectively. Compared with the control group, curcumol significantly inhibited the in vitro migration and invasion of MG-63 and U2OS cells (P<0.05) and induced the cell apoptosis (P<0.05). Compared with the control group, expressions of Bax and caspase 3 were promoted while expression of Bcl2 was inhibited in curcumol groups (P<0.05). The mRNA and protein expressions of β-catenin in MG-63 and U2OS cells were signifi- cantly inhibited (P<0.05). Conclusions Curcumol significantly inhibits proliferation and induces apoptosis of human osteosarcoma cell lines MG-63 and U2OS.

Key words: Curcumol, β-catenin, osteosarcoma, cell proliferation, cell apoptosis

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