Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (7): 1031-1034.doi: 10.16352/j.issn.1001-6325.2022.07.1031

• Original Articles • Previous Articles     Next Articles

Application of a new micro-cannula for the establishment of a Parkinsonian mouse model

ZUO Fu-xing1, YUAN Qing1, CAI Hong-qing1, HAN Rui-qin2*, WAN Jing-hai1   

  1. 1. Department of Neurosurgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, CAMS & PUMC, Beijing 100021;
    2. State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China
  • Received:2022-05-07 Revised:2022-05-19 Online:2022-07-05 Published:2022-06-29
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Abstract: Objective To explore the feasibility of a modified micro-cannula for intra-cerebral administration of 6-hydroxydopamine(6-OHDA) in order to establish a Parkinsonian mouse model. Methods Twenty female mice(C57BL/6J) were randomly divided into 2 groups with 10 in each: experimental group (new micro-cannula) and control group (regular Hamilton syringe). Following unilateral injection of 6-OHDA, the behavioral test and histological microscopy were performed. Results All mice in experimental group showed the morphology of apomorphine-induced rotational asymmetry, but the same results were found in only 5 mice from control group. Total number of rotations away from the lesion side in 30 minutes showed 6-OHDA-induced motor deficits at day 7 in both groups, which was significantly attenuated at day 28 in control group (P<0.05). After 28 days of 6-OHDA injection, significant reduction in the number of rotations was observed in control mice as compared with that of experimental group (P<0.05). As immunohistochemistry showed that the total number of remaining dopaminergic neurons significantly decreased in the substantial nigra of the experimental mice as compared with the control mice(P<0.05). Moreover, more local astrocytes underwent activation, part of which acquired the properties of neural precursor cells in control mice. Conclusions Intrastriatal injection of 6-OHDA using the new micro-cannula is a feasible technology in the development of a more reliable Parkinsonian model of mice.

Key words: Parkinson's disease, 6-hydroxydopamine(6-OHDA), stereotactic frame, new micro-cannula, dopaminergic neurons

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