Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (6): 927-932.doi: 10.16352/j.issn.1001-6325.2022.06.008

• Original Articles • Previous Articles     Next Articles

Okadaic acid induces damage of human neuroblastoma cell line SH-SY5Y

LU Ya-lan, ZHOU Li, HAN Yun-lin, WANG Ke-wei, QIN Chuan*   

  1. Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences; Comparative Medicine Center, Peking Union Medical College; Key Laboratory of Human Disease Comparative Medicine, National Health Commission, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Disease, Beijing 100021, China
  • Received:2021-05-24 Revised:2022-03-28 Online:2022-06-05 Published:2022-06-02
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Abstract: Objective To investigate the effect of okadaic acid (OA)-induced phosphorylation of neuron micro-tubule associated protein tau (Tau) on the damage of human neuroblastoma SH-SY5Y cells. Methods SH-SY5Y cells were incubated with different concentrations (20 and 40 nmol/L) of OA for 0, 12, 24, 48 and 72 hours followed by microscopy for cell morphology.Cell ultrastructure was observed by transmission electron microscope and cell apoptosis was detected by TUNEL assay. The protein expression of p-Tau and caspase3 was analyzed by Western blot and mRNA level of inhibitor of apoptosis family proteins (IAPs) was determined by RT-qPCR. Results OA induced phosphorylation of Tau protein in SH-SY5Y cells as compared to the result from control goup (P<0.001), promoted cell damage and apoptosis (P<0.001).OA also injured mitochondrial structure in a concentration and time dependent manner. It can also up-regulate IAPs expression significantly (P<0.05). Conclusions The aberrant phosphorylation of Tau protein in SH-SY5Y cells induced by OA may lead to cell injury and apoptosis. Inhibition of endogenous apoptotic pathways or up-regulation of IAPs family proteins provides potential targets for AD prevention and treatment.

Key words: Alzheimer's disease, okadaic acid, Tau, neurofibrillary tangle

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