Abstract: Objective To explore the function of circ-MALAT1 targeting miR-101 in the inhibition of the invasion and migration of bladder cancer cells. Methods Bladder cancer cell line SW780 was divided into si-NC group (transfection si-NC), si-circ-MALAT1 group (transfection si-circ-MALAT1), miR-NC group (transfection miR-NC), miR-101 group (transfection miR-101 mimics), si-circ-MALAT1+anti-miR-NC group(co-transfection si-circ-MALAT1 and anti-miR-NC)and si-circ-MALAT1+anti-miR-101 group(co-transfection si-circ-MALAT1 and anti-miR-101). RT-qPCR was used to examine the expression of circ-MALAT1 and miR-101; Transwell method was used to detect cell invasion and migration; The matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9),phosphorylated phosphatidylinositol 3-kinase (p-PI3K), phosphorylated protein kinase B(p-AKT) protein expression were measured by Western blot; Dual luciferase reporter gene experiment was applied to detect the targeting relationship between circ-MALAT1 and miR-101. Results Compared with adjacent tissues, the expression of circ-MALAT1 in bladder cancer tissue was significantly increased (P＜0.05)and the expression of miR-101 was significantly decreased (P＜0.05). Compared with the si-NC group, the expression of circ-MALAT1 in the si-circ-MALAT1 group was significantly reduced(P＜0.05)and the counting number of invasion and migrating cells was significantly reduced (P＜0.05), the expression of p-PI3K and p-AKT protein was significantly reduced(P＜0.05). Compared with the miR-NC group, the expression of miR-101 in the miR-101 group was significantly increased(P＜0.05)and the number of invasion as well as migrating cells were significantly reduced(P＜0.05).Compared with the (si-circ-MALAT1+anti-miR-NC)group, the(si-circ-MALAT1+anti-miR-101)group showed more cell invasion and migration (P＜0.05), the expression of p-PI3K and p-AKT protein was significantly increased(P＜0.05). Conclusions Reduction of circ-MALAT1 expression and up-regulation miR-101 inhibit the invasion and migration of bladder cancer cells line SW780. The mechanism may be related to the PI3K/AKT signaling pathway.

Key words: circ-MALAT1, miR-101, bladder cancer, PI3K/AKT signaling pathway, invasion