RNA binding protein PCBP1 regulates hESC pluripotency through modulating mRNA export

doi:10.16352/j.issn.1001-6325.2022.02.027

Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (2): 228-234.doi: 10.16352/j.issn.1001-6325.2022.02.027

• Original Articles • Previous Articles Next Articles

RNA binding protein PCBP1 regulates hESC pluripotency through modulating mRNA export

XU Jia-yue, YANG Jia-bin, CHEN Zhong-yang, YU Jia, MA Yan-ni^*

State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medicine CAMS, School of Basic Medicine PUMC, Beijing 100005, China

Received:2021-10-11 Revised:2021-12-20 Online:2022-02-05 Published:2022-01-24
Contact: ^* yanni_ma@126.com

Abstract

Abstract: Objective To investigate the biological function of RNA binding protein polycytosine-binding protein 1 (PCBP1) in the maintenance of pluripotency of human embryonic stem cells (hESC) and to explore the new mechanism of regulating hESC pluripotency. Methods PCBP1 knockdown technology was used to detect the clonogenicity. The alkaline phosphatase staining profile and the expression of pluripotency/differentiation marker genes were detected by qPCR. Meanwhile, the nucleus and cytoplasm of hESC were isolated for RNA sequencing, and the nucleoplasmic changes of RNA generated by PCBP1 knockdown were analyzed by multi-level analysis. Results PCBP1 knockdown significantly inhibited clonogenesis of human embryonic stem cells (P＜0.01) and reduced the RNA level of pluripotency related genes (P＜0.01). The expression of differentiation related genes increased(P＜0.01)that significantly affected the overall nucleoplasmic distribution of RNA in hESC and the nucleoplasmic distribution of mRNA related to embryonic development and cell differentiation. Conclusions PCBP1 regulates hESC pluripotency by regulating the expression of pluripotency/differentiation marker genes and the nucleoplasmic localization of mRNA.

Key words: human embryonic stem cell, polycytosine binding protein 1, pluripotency and self-renewal, nuclear and cytoplasm extraction

CLC Number:

XU Jia-yue, YANG Jia-bin, CHEN Zhong-yang, YU Jia, MA Yan-ni. RNA binding protein PCBP1 regulates hESC pluripotency through modulating mRNA export[J]. Basic & Clinical Medicine, 2022, 42(2): 228-234.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2022.02.027

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2022/V42/I2/228

References

[1]Weatherbee BAT, Cui T, Zernicka-Goetz M. Modeling human embryo development with embryonic and extra-embryonic stem cells[J]. Dev Biol, 2021,474:91-99.
[2]Castello A, Fischer B, Eichelbaum K, et al. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins[J]. Cell, 2012, 149:123-128.
[3]Hafner M, Landthaler M, Burger L, et al. Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP[J]. Cell, 2010, 141:129-141.
[4]Wickramasinghe VO, Laskey RA. Control of mammalian gene expression by selective mRNA export[J]. Nat Rev Mol Cell Biol, 2015, 16:431-442.
[5]Meng Q, Rayala SK, Gururaj AE, et al. Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1[J]. PNAS, 2007, 104:5866-5871.
[6]Fujimura K, Katahira J, Kano F, et al. Selective localization of PCBP2 to cytoplasmic processing bodies[J]. Biochim Biophys Acta, 2009, 1793:878-887.
[7]Ghanem LR, Kromer A, Silverman IM, et al. The poly(C) binding protein pcbp2 and its retrotransposed derivative pcbp1 are independently essential to mouse development[J]. Mol Cell Biol, 2015,36:304-319.
[8]Ryu MS, Zhang D, Protchenko O, et al. PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis[J]. J Clin Invest, 2017, 127:1786-1797.
[9]Geuens T, Bouhy D, Timmerman V. The hnRNP family: insights into their role in health and disease[J]. Human Genetics, 2016, 135:851-867.
[10]Thorvaldsen JL, Duran KL, Bartolomei MS. Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2[J]. Genes Dev, 1998, 12:45-48.
[11]Engreitz JM, Pandya-Jones A, Mcdonel P, et al. The Xist lncRNA exploits three-dimensional genome architec-ture to spread across the X Chromosome[J]. Science, 2013, 341:767-769.
[12]Segref A, Sharma K, Doye A, et al. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores[J]. EMBO J, 2014, 16:3256-3271.
[13]Cullen BR. Nuclear mRNA export: insights from virology[J]. TIBS, 2003, 28:419-424.
[14]Luo ML, Zhou Z, Magni K, et al. Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly[J]. Nature, 2001, 413:644-647.

RNA binding protein PCBP1 regulates hESC pluripotency through modulating mRNA export

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	. hnRNPA3 regulates hESC pluripotency through modulating mRNA export [J]. Basic & Clinical Medicine, 2021, 41(6): 811-817.
[2]	Meng-Yao SUN siqi liu Fan-Qi ZHOU Ja -YU. Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells [J]. Basic & Clinical Medicine, 2021, 41(5): 636-640.
[3]	. RNA localization in cytoplasm and nucleus of human embryonic stem cells [J]. Basic & Clinical Medicine, 2020, 40(6): 765-770.