Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (2): 215-220.doi: 10.16352/j.issn.1001-6325.2022.02.002

• Original Articles • Previous Articles     Next Articles

Identification of differentially expressed genes in cervical carcinoma after radiotherapy

BAI Jing1#*, LIU Na2,3,4#, DING Li1, LEI Li1, NI Rui1, YAN Shao-chun2*, SHAO Guo2,3,4*   

  1. 1. Department of Radiptherapy, Baotou Tumor Hospital,Baotou 014030;
    2. Inner Mongolia Key laboratory of Hypoxic Translational Medicine,Baotou Medical College,Baotou 014060;
    3. Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital,Capital Medical University,Beijing 100053;
    4. Institute of Digestive Diseases of Inner Mongolia Autonomous Region, Department of Laboratory Medicine, the Second Affiliated Hospital of Baotou Medical College,Baotou 014030,China
  • Received:2020-11-13 Revised:2021-06-08 Online:2022-02-05 Published:2022-01-24
  • Contact: *;;

Abstract: Objective To study the effect of radiotherapy on gene expression in cervical cancer tissues and to find the role of differentially expressed genes in sensitivity and resistance to radiotherapy. Methods The patients all received the same radiotherapy as follows: total pelvic external radiation 45 Gy/25 f,intracavitary brachytherapy 5-6 Gy/f with total dosage as 5-6 f.The specimens were collected before treatment and by the end of treatment.High-throughput analysis of gene expression was performed before, during and after irradiation; real-time PCR and Western blot was applied to identify the genes related to radiation sensitivity and tolerance. Results Bioinformatics analysis showed an increased expression of DNA damage genes.The expression of DNA damage related genes GADD45 was increased as found by real-time PCR and Western blot. Conclusions Radiotherapy changes the gene expression in cervical cancer tissues and the increase of GADD45 expression may play an important role in radiotherapy.

Key words: cervical cancer, radiotherapy, bioinformatics

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