癌基因DEK和转录因子AP-2α协同促进乳腺癌中HER2的高表达

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (2): 175-178.

癌基因DEK和转录因子AP-2α协同促进乳腺癌中HER2的高表达

黄前川曹军皓季蒙

广州军区武汉总医院

收稿日期:2009-02-11 修回日期:2009-06-22 出版日期:2010-02-05 发布日期:2010-02-05
通讯作者: 黄前川

Oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer

Qian-chuan HUANG, Jun-hao CAO, Meng JI

Wuhan General Hospital of Guangzhou Command

Received:2009-02-11 Revised:2009-06-22 Online:2010-02-05 Published:2010-02-05
Contact: Qian-chuan HUANG,

摘要/Abstract

摘要： 目的探讨癌基因DEK与转录因子AP-2α在乳腺癌变中的相互作用及在HER2过量表达、乳腺癌变中的病理学意义。方法用Western blot检测组织中DEK、AP-2α和HER2蛋白水平之间的相关性；免疫共沉淀检测DEK和AP-2α在MDA-MB-453乳腺癌细胞中的相互作用；通过siRNA抑制MDA-MB-453细胞中DEK和AP-2α的表达，用半定量RT-PCR 和Western blot检测HER2的表达。结果乳腺癌组织中DEK、AP-2α和HER2的蛋白水平之间显示一定的相关性, DEK和AP-2α在MDA-MB-453细胞内有相互作用,siRNA抑制DEK和AP-2α的表达可协同抑制MDA-MB-453细胞中HER2 mRNA和蛋白的表达。结论本研究证明癌基因DEK和转录因子AP-2α协同促进乳腺癌中HER2的高表达。

关键词: DEK, AP-2α, HER2, 乳腺癌

Abstract: Objective To investigate the pathological significance of DEK-AP-2α interaction in HER2 overexpression and breast tumorigenesis. Methods The protein level of DEK，AP-2α and HER-2 in breast tumor tissues was detected by Western blot. The interaction of DEK and AP-2α in MDA-MB-453 mammary cancer cells was detected by immuno-coprecipitation. Furthermore the impact of DEK and AP-2α on HER2 expression was investigated by siRNA in MDA-MB-453 mammary cancer cells followed by semi-quantitive RT-PCR and Western blot. Resluts A correlation between DEK, AP-2α and HER2 levels in breast cancer tissues were observed. The interaction between DEK and AP-2α in MDA-MB-453 cells was verified by co-immunoprecipitation assay. Depletion of DEK and AP-2α in MDA-MB-453 cell by siRNA cooperatively repressed HER2 expression at both the mRNA and protein levels. Conclution This study provides oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer.

Key words: DEK, AP-2α, HER2, breast cancer

黄前川曹军皓季蒙. 癌基因DEK和转录因子AP-2α协同促进乳腺癌中HER2的高表达[J]. 基础医学与临床, 2010, 30(2): 175-178.

Qian-chuan HUANG; Jun-hao CAO; Meng JI. Oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer[J]. Basic & Clinical Medicine, 2010, 30(2): 175-178.

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