次级淋巴组织趋化因子浓度梯度抑制肿瘤细胞免疫逃逸

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (8): 846-848.

次级淋巴组织趋化因子浓度梯度抑制肿瘤细胞免疫逃逸

吴砂袁晓梅朱慧芬沈关心

武汉华中科技大学同济医学院免疫学系武汉华中科技大学同济医学院免疫学系华中科技大学同济医学院免疫学研究所华中科技大学同济医学院免疫学研究所

收稿日期:2007-04-11 修回日期:2007-04-24 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 吴砂

Effects of SLC concentration gradient on suppression of tumor immune escape

Sha Wu Xiao-mei Yuan Hui-fen Zhu Guan-xin Shen

Received:2007-04-11 Revised:2007-04-24 Online:2007-08-25 Published:2007-08-25
Contact: Sha Wu

摘要/Abstract

摘要： 目的探讨人次级淋巴组织趋化因子(SLC)浓度梯度对肿瘤细胞免疫逃逸的影响。方法根据SLC浓度梯度，实验分6组；流式细胞术检测肿瘤细胞HLA-Ⅰ类分子的表达、肿瘤细胞凋亡，Western blot检测肿瘤细胞胞内Bcl-2表达，ELISA检测肿瘤细胞培养上清中生长转化因子β(TGF-β)水平。结果在一定浓度范围内，随SLC浓度增加，肿瘤细胞的HLA-Ⅰ类分子表达增高，肿瘤细胞凋亡增多； Bcl-2表达增高，而肿瘤细胞TGF-β分泌水平降低。结论 SLC具有抑制肿瘤细胞免疫逃逸的作用。

Abstract: Objective The aim of this study was to explore the effects of SLC concentration gradient on suppression of tumor immune escape. Methods According to different SLC concentration, there were six groups. The HLA-Ⅰexpression and apoptosis of MCF-7 were detected with FCM ,and intracellular Bcl-2 expression was analyzed by western blot. The productions of TGF-βwere detected with ELISA. Results In a certain range of concentration gradient, following SLC increase, HLA-Ⅰexpression level on MCF-7 was improved, and the apoptosis was induced but Bcl-2 expression was enhanced ,moreover, the secretion of TGF-β was suppressed. Conclusion SLC inhibited tumor immune escape.

吴砂袁晓梅朱慧芬沈关心. 次级淋巴组织趋化因子浓度梯度抑制肿瘤细胞免疫逃逸[J]. 基础医学与临床, 2007, 27(8): 846-848.

Sha Wu Xiao-mei Yuan Hui-fen Zhu Guan-xin Shen. Effects of SLC concentration gradient on suppression of tumor immune escape[J]. Basic & Clinical Medicine, 2007, 27(8): 846-848.