Ghrelin 抑制小鼠骨髓基质细胞成脂分化

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (8): 886-890.

Ghrelin 抑制小鼠骨髓基质细胞成脂分化

周华杨曦张雅鸥蔡国平

清华大学生物科学与技术系；清华大学深圳研究生院生命学部第四军医大学遗传与发育生物学教研室清华大学生物科学与技术系；清华大学深圳研究生院生命学部清华大学生物科学与技术系；清华大学深圳研究生院生命学部

收稿日期:2006-08-30 修回日期:2006-10-18 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 蔡国平

Inhibitory effect of ghrelin on adipocytic differentiation

Hua Zhou Xi Yang Ya-ou Zhang Guo-ping Cai

Received:2006-08-30 Revised:2006-10-18 Online:2007-08-25 Published:2007-08-25
Contact: Guo-ping Cai

摘要/Abstract

摘要： 观察胃促生长素ghrelin 对原代培养小鼠骨髓基质细胞成脂分化的影响。方法体外培养小鼠骨髓基质细胞， MDI诱导剂诱导细胞成脂分化，显微镜观察细胞形态变化；油红O染色法检测细胞甘油三酯含量，化学比色法测定碱性磷酸酶(ALP)活性；MTT法检测细胞克隆化扩增活动，RT-PCR检测过氧化物酶体增殖物激活受体γ2(PPARγ2)和丝氨酸蛋白酶脂肪因子adipsin mRNA表达，Western blot 检测PPARγ2蛋白表达。结果 10-9、10 -8、10-7 mol/L ghrelin能够剂量依赖性的抑制骨髓基质细胞成脂分化，升高ALP活性(P<0.05或P <0.01)。ghrelin能够降低PPARγ2和adipsin mRNA以及PPARγ2蛋白表达(P<0.05)。ghrelin对细胞成脂分化早期的克隆化扩增没有显著影响。结论 ghrelin能够显著抑制体外培养小鼠骨髓基质细胞成脂分化，该作用可能与抑制PPARγ2表达有关。

关键词: ghrelin, 骨髓基质细胞, 成脂分化

Abstract: objective: To investigate the effect of ghrelin on adipocytic differentiation of bone marrow stromal cells (BMSCs) .Methods: BMSCs from mice were cultured , and adipocytic differentiation was induced by MDI inducers .The morphological changes of the cells were observed .Oil-Red O staining measurement , alkaline phosphatase (ALP) activity measurement ,MTT assay ,RT-PCR and Western blot were employed . Results: 10-9，10 -8，10-7 mol/L ghrelin could dose-dependently inhibit the adipocytic differentiation of BMSCs and promote ALP activity (P<0.05 or P <0.01). Ghrelin decreased the expression of PPARγ2, adipsin mRNA and PPARγ2 protein. Ghrelin exerted no influences on clonal expansion in early phase of differentiation. Conclusion：ghrelin can inhibit the adipocytic differentiation of BMSCs ,which might be closely related to the downregulation of PPARγ2.

Key words: ghrelin, bone marrow stromal cells, adipocytic differentiation

周华杨曦张雅鸥蔡国平. Ghrelin 抑制小鼠骨髓基质细胞成脂分化[J]. 基础医学与临床, 2007, 27(8): 886-890.

Hua Zhou Xi Yang Ya-ou Zhang Guo-ping Cai. Inhibitory effect of ghrelin on adipocytic differentiation[J]. Basic & Clinical Medicine, 2007, 27(8): 886-890.

[1]	黄姗王世华韩钦赵春华. miR-424负向调节人脂肪源间充质干细胞向成脂细胞的分化[J]. 基础医学与临床, 2012, 32(2): 114-118.
[2]	徐建平杨保卫. Ghrelin对心衰恶液质的治疗作用*[J]. 基础医学与临床, 2012, 32(1): 102-105.
[3]	杨志军罗永春徐如祥柯以铨姜晓丹蔡颖谦杜谋选邹雨汐. 菲立磁胞内标记食蟹猴骨髓基质细胞[J]. 基础医学与临床, 2009, 29(8): 859-862.
[4]	周辉姜晓丹徐如祥蔡颖谦邹雨汐. 绿色荧光蛋白转基因小鼠骨髓基质细胞体外诱导分化为神经元样细胞[J]. 基础医学与临床, 2009, 29(6): 566-569.
[5]	段春礼姬曼孙晓红赵春礼杨慧. 大鼠骨髓基质细胞在脑内的迁移和分化[J]. 基础医学与临床, 2008, 28(5): 432-435.