脂质体转染VEGF-C反义寡核苷酸对人胃癌OCUM-2MD3细胞表达的影响

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (2): 148-151.

脂质体转染VEGF-C反义寡核苷酸对人胃癌OCUM-2MD3细胞表达的影响

李勇杨进强赵增仁

河北医科大学第四医院外科河北医科大学第四医院外科河北医科大学第四医院外科

收稿日期:2005-07-22 修回日期:2006-08-28 出版日期:2007-02-25 发布日期:2007-02-25
通讯作者: 杨进强

Effect of VEGF-C-ASODN on VEGF-C expression in human gastric cancer cell OCUM-2MD3 induced by liposome

Received:2005-07-22 Revised:2006-08-28 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： 目的探讨脂质体转染VEGF-C反义寡核苷酸在人胃癌OCUM-2MD3细胞中的转染效率及对VEGF-C表达的影响。方法以VEGF-C基因翻译起始区为靶点合成反义寡核苷酸，脂质体介导转染人胃癌OCUM-2MD3细胞，荧光显微镜观察转染效率，RT-PCR检测转染后VEGF-CmRNA表达水平，流式细胞术检测VEGF-C蛋白表达。结果经脂质体介导可成功将VEGF-C反义寡核苷酸转染至胃癌OCUM-2MD3细胞，12h达其峰值87.93%。反义组VEGF-CmRNA相对吸光度值为0.38±0.11，显著低于正义组(0.83±0.17)和对照组(0.80±0.14) (P<0.01)。反义组VEGF-C蛋白表达水平(31.26±5.17)%，较正义组(41.83±7.49)%和对照组(38.65±7.11)%亦明显下降(P<0.01)。结论 VEGF-C反义寡核苷酸经脂质体介导转染胃癌OCUM-2MD3细胞，可降低胃癌细胞VEGF-CmRNA及其蛋白表达水平。

Abstract: Objective To investigate the influence of VEGF-C antisense oligodeoxynuclecotide (ASODN) on the VEGF-C expression and transfection effects in human gastric cancer cell line OCUM-2MD3 induced by liposome. Methods The ASODN sequence, which was synthesized according to the coding region of VEGF-C gene, was transformed into human gastric cancer cell line OCUM-2MD3 induced by liposome. The transfection effects were observed by fluorescence microscopy. The changes in the expression of VEGF-C mRNA and protein were determined by RT-PCR and flow cytometry(FCM)，respectively. Results VEGF-C-ASODN could be successfully transformed into gastric cancer cell induced by liposome, and its optimal ratio was 87.93% after 12 hours. Compared with the control and oligodeoxynuclecotide (SODN) group, not only was the VEGF-C mRNA level (Ar=0.38±0.11) significantly decresaed(P<0.01), but also the protein of VEGF-C (31.26±5.17)% was dramatically lowed(P<0.01). Conclusion VEGF-C-ASODN that was transformed into human gastric cancer cell induced by liposome may inhibit the expression of VEGF-C mRNA and protein.

李勇杨进强赵增仁. 脂质体转染VEGF-C反义寡核苷酸对人胃癌OCUM-2MD3细胞表达的影响[J]. 基础医学与临床, 2007, 27(2): 148-151.

. Effect of VEGF-C-ASODN on VEGF-C expression in human gastric cancer cell OCUM-2MD3 induced by liposome[J]. Basic & Clinical Medicine, 2007, 27(2): 148-151.