基础医学与临床 ›› 2008, Vol. 28 ›› Issue (7): 734-737.
• 研究论文 • 上一篇 下一篇
陈佩林
收稿日期:
修回日期:
出版日期:
发布日期:
通讯作者:
Pei-lin CHEN
Received:
Revised:
Online:
Published:
Contact:
摘要： 目的 探讨CCNH基因启动子区- 1SNP位点碱基变异对CCNH基因转录活性的影响。方法 通过PCR和定点突变技术,构建了含CCNH基因基础启动子及- 1G突变启动子,用双荧光素酶报告系统观察此SNP对CCNH基因启动子转录活性的影响。结果 在AD293细胞中, -1G突变启动子活性比CCNH基础启动子活性低26%（P<0.05）。结论 -1T→G碱基变异可显著降低CCNH基因启动子转录活性。
关键词: CCNH基因, SNP, 双荧光素酶报告系统
Abstract: Objective To study the effect of -1 site single nucleotide polymorphism ( SNP) on CCNH gene promoter transcription activity. Methods PCR and site-directed mutagenesis technology were used to construct CCNH basic promoter and -1G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the transcription activity of constructed promoter. Results In AD293 cells, the activity of - 1G mutate type promoter was significantly lower than wild type -1T promoter（P<0.05）. Conclusion It is implied that SNP-1T→G base variation can decrease the transcription activity of CCNH gene promoter.
Key words: CCNH gene, SNP, Dual-Luciferase Reporter assay system
陈佩林. Cyclin H基因（CCNH）启动区SNP对转录活性的影响[J]. 基础医学与临床, 2008, 28(7): 734-737.
Pei-lin CHEN. Effect of - 1 SNP on CCNH promoter activity[J]. Basic & Clinical Medicine, 2008, 28(7): 734-737.
0 / / 推荐
导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks
链接本文: http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2008/V28/I7/734
