关键词: 原发性高血压, PCR-RFLP, α-Adducin, GNB3, 基因多态性

Abstract: Objective To explore the polymorphism at position G460W of α-ADDUCIN and at position C825T of GNB3 and the genetic interaction between α-ADDUCIN and GNB3 genes in a QiQihr essential hypetension population. Methods Three hundreds and thirty-one patients with EH and two hundreds and ninety-three healthy controls were selected．Genotyping was performed using PCR-RFLP technique. Results (1)genotype distributions of alpha- ADDUCIN G460W (GG 0.177 vs 0.160, GW 0.580 vs 0.481, WW 0.242 vs 0.359, P=0.006) and GNB3 (CC0.177 vs 0.353, CT 0.468 vs 0.541, TT 0.355 vs 0.106, P＜0.001) between NT group and EH group; there were significantly differences in W allele frequency of alpha-ADDUCIN G460W ( 0.532 vs 0.600, P=0.017) between two group. (2) GNB3 (CC0.177 vs 0.353, CT0.468 vs 0.541, TT0.355 vs 0.106, P＜0.001) between NT group and EH group.; there were significantly different in T allele frequency of GNB3 C825T (0.589 vs 0.376, P＜0.001) between two group; (3)The genotype distributions of WW+CC and WW+CT of the EH group were significantly higher than the NT group (P value respectively ＜0.001 and＜0.01), yet the genotypes distribution of GG+TT、GW+TT and WW+TT of the EH group were significantly lower than the NT group (P value respectively ＜0.01、＜0.001 and ＜0.001). Conclusion These observations suggested that alpha-ADDUCIN G460W and GNB3 C825T polymorphisms are associated with essential hypertension in QiQihr population, and there might be an interacting effect between the two genes.

Key words: Essential Hypetension, PCR-RFLP, α-Adducin, GNB3, Polymorphism