Abstract: Objectives To study the potential of hepatocyte growth factor (HGF), acid fibroblast growth factor (aFGF) and oncostatin M (OSM) to induce MSCs differentiate into hepatocytes and their roles. Methods MSCs were treated with HGF, aFGF and OSM. Detecting the expression of AFP, ALB and urea was performed by chemi-luminescent technique, RT-PCR method and glutamic acid dehydrogenase technique. Results AFP expression emerged in every group except control group (P<0.05). ALB and urea expression emerged in other groups except control group and a+O group (P<0.05). Conclusion HGF, OSM and aFGF can induce MSCs differentiate into hepatocytes with the expression of AFP, ALB and excreting urea, while aFGF and OSM can not induce MSCs differentiate into mature hepatocyte without HGF.