临床宫颈组织标本HPV16 L1蛋白检测

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (8): 807-810.

临床宫颈组织标本HPV16 L1蛋白检测

时艳梅肖长义叶红王雅琴吴江锋

三峡大学医学院三峡大学第一临床医学院

收稿日期:2009-04-27 修回日期:2009-11-12 出版日期:2010-08-05 发布日期:2010-08-05

The Detection of HPV16 L1 in Infected Cervical Specimen

Yan-mei SHI, Chang-yi XIAO, Hong YE, Ya-qin WANG, Jiang-feng WU

Medical College of China Three Gorges University

Received:2009-04-27 Revised:2009-11-12 Online:2010-08-05 Published:2010-08-05

摘要/Abstract

摘要： 目的检测HPV16阳性宫颈标本L1蛋白的表达，发掘宫颈损伤程度与L1蛋白表达的规律。方法 PCR法对宫颈炎、宫颈上皮内瘤变（CINⅠ~Ⅱ）、原位癌、早期浸润癌及中晚期癌标本进行型别检测，ELISA法及免疫组化法检测HPV16阳性标本中HPV L1蛋白表达。结果 54例宫颈组织中HPV16阳性为46例（85.2%）。随着宫颈损伤加重，HPV16阳性标本的抗原抗体反应逐渐减弱；免疫组化阳性反应仅出现于上皮组织内，反应强度及分布与宫颈损伤程度及癌变组织的分化状态有关。结论 HPV16 L1蛋白的表达同时受宫颈组织损伤程度及宿主细胞分化状态的影响。早期对HPV L1蛋白进行检测，可为宫颈癌的早期预防及临床诊治提供理论指导。

关键词: 人乳头瘤病毒, 宫颈癌, 衣壳蛋白L1, 聚合酶链式反应, 免疫组化

Abstract: Objectives Detecting the expression of L1 in HPV16 infected cervical tissues and unearthing the correlation of HPV16 L1 with cervix injured degree. Methods Multiplex PCR was used to detect HPV16-positive specimen in cervicitis, CINⅠ~Ⅱ,cancer in situ and cervical cancer. The expression of L1 was detected in each group by ELISA and Immunohistochemistry. Results 46 out of 54 cases were HPV16 positive(85.2%). The reaction between antigen and antibody was lower as the aggravated of cervical damage in ELISA; the positive reaction appeared in the epithelium tissue in Immunohistochemistry. which effected by tissue`s damaged degree and differentiated states. Conclusion There are higher rate of HPV16 detection in women. L1 expressed in epithelium which affected by tissue`s differentiation. The expression of HPV16 L1 was lower as the damage aggravated. Detecting HPV L1 earlier in clinic can provide the direction for cervical cancer.

Key words: human papillomavirus, cervical carcinoma, L1 capsid protein, PCR, Immunohistochemistry

中图分类号:

R737.33

时艳梅肖长义叶红王雅琴吴江锋. 临床宫颈组织标本HPV16 L1蛋白检测[J]. 基础医学与临床, 2010, 30(8): 807-810.

Yan-mei SHI; Chang-yi XIAO; Hong YE; Ya-qin WANG; Jiang-feng WU. The Detection of HPV16 L1 in Infected Cervical Specimen[J]. Basic & Clinical Medicine, 2010, 30(8): 807-810.

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