FADDDEL-GFP基因修饰在小鼠胰岛细胞移植中的作用

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 375-378.

FADDDEL-GFP基因修饰在小鼠胰岛细胞移植中的作用

胡萍(广州）吴砂（广州）李小兰韦静

暨南大学医学院微生物学和免疫学教研室南方医科大学免疫教研室

收稿日期:2007-10-10 修回日期:2008-07-08 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 胡萍(广州）

Effect of FADDDEL-GFP modification in the islet cell transplantion

Ping HU, Sha WU, Xiao-lan LI, Jing WEI

Dept.of Microbiology and Immunology, Medical College, Jinan University Dept.of Immunology, Nanfang Medical University

Received:2007-10-10 Revised:2008-07-08 Online:2009-04-25 Published:2009-04-25
Contact: Ping HU,

摘要/Abstract

摘要： 目的探讨融合蛋白FADDDEL-GFP在胰岛细胞移植治疗1型糖尿病中的作用。方法将重组子FADDDEL-GFP导入胰岛细胞NIT-1，检测基因转染前后胰岛细胞分泌胰岛素的水平。用STZ诱导1型糖尿病模型小鼠，将FADDDEL-GFP修饰的NIT-fg细胞移植于糖尿病小鼠，检测胰岛细胞移植后对糖尿病小鼠的影响。结果转染重组子FADDDEL-GFP的NIT-1可见绿色荧光蛋白表达；移植NIT-fg细胞的糖尿病小鼠血糖降至正常，生存期延长，与对照组有明显差异。结论FADDDEL-GFP可增强机体抗同种移植排斥反应的能力。

Abstract: Objective To study the effect of fusion protein FADDDEL-GFP in the treatment of type 1 diabetes by islet cell transplantion. Methods After transfecting the recombinate FADDDEL-GFP into mouse insulinoma cells NIT-1, insulin level secreted by the cells was detected. Type 1 diabetes model was induced by STZ. NIT-fg cells, the NIT-1 cells modified by FADDDEL-GFP, were transplanted into the diabetic mice. Then the effects of islet cell transplantation on diabetic mice were detected. Results GFP was expressed in NIT-1 cells transfected with FADDDEL-GFP. Diabetic mice reached normoglycemia and prolonged the life span, which is different obviously from the control group. ConclusionsFADDDEL-GFP could enhance the capability of resisting allogeneic transplantation rejection.

胡萍(广州）吴砂（广州）李小兰韦静. FADDDEL-GFP基因修饰在小鼠胰岛细胞移植中的作用[J]. 基础医学与临床, 2009, 29(4): 375-378.

Ping HU; Sha WU; Xiao-lan LI; Jing WEI. Effect of FADDDEL-GFP modification in the islet cell transplantion[J]. Basic & Clinical Medicine, 2009, 29(4): 375-378.