利用蛋白质芯片技术筛选及确定白血病血清标志蛋白质

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (3): 333-336.

利用蛋白质芯片技术筛选及确定白血病血清标志蛋白质

张军张建中王惠芳马一盖王建文

北京药物化学研究所北京药物化学研究所北京药物化学研究所

收稿日期:2006-09-27 修回日期:1900-01-01 出版日期:2007-03-25 发布日期:2007-03-25
通讯作者: 张军

Screening of serum biomarkers in leukemia bu SELDI technique:a preliminary study

Received:2006-09-27 Revised:1900-01-01 Online:2007-03-25 Published:2007-03-25

摘要/Abstract

摘要： 目的利用蛋白质芯片从初诊急性白血病患者血清中筛选标记蛋白质。方法采用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS surfaced enhanced laser desorption／ionization time of flight mass spectroscopy)和弱阳离子交换（WCX2）蛋白质芯片对80例初诊急性白血病患者(44例儿童急性淋巴细胞白血病、36例成人急性粒细胞白血病)血清和78例正常人（35例为儿童）血清的蛋白质图谱进行检测，使用PBSII-C型蛋白质芯片阅读机读取数据，以P<10-5时具有统计学意义。结果急性淋巴细胞白血病初诊患儿与正常儿童血清蛋白质谱相比有显著差异蛋白质7个，用标记分子建立儿童急性淋巴细胞白血病诊断的分类树模型，检测灵敏度为88.6％（39/44）、特异性为82.9％（29/35）；成人急性粒细胞白血病患者与正常成人血清蛋白质谱相比有显著差异蛋白质8个，用其中的7个标记分子建立成人急性粒细胞白血病诊断的分类树模型，检测灵敏度为86.1％（31/36）、特异性为95.3％（41/43）。结论蛋白质芯片技术对于发现和筛选急性白血病血清中的标记蛋白质是一种有效、快速的工具。

Abstract: Objective The aim of this study was to screen serum biomarkers in patients with acute leukemia by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique. Methods Proteomic spectra were generated by mass spectrometry in 158, including 44 acute lymphocyte leukemia patients and 36 acute myeloid leukemia, 78 healthy control. 158 spectra obtained were used to train and develop a decision tree classification algorithm. Results A total of 7 distinguished proteomic peaks were detected in acute lymphocyte leukemia and were used to build a proteomic pattern. The results yielded a sensitivity of 88.6％（39/44）、specificity of 82.9％（29/35）；A total of 8 distinguished proteomic peaks were detected in acute myeloid leukemia，7 peaks were used to build a proteomic pattern. The results yielded a sensitivity of 86.1％（31/36）、specificity of 95.3％（41/43）. Conclusion SELDI-TOF-MS offers a unique platform for the proteomic detection of acute leukemia. It also offers a noninvasive method to further study the proteomic changs in the development and progression of leukemia.

张军张建中王惠芳马一盖王建文. 利用蛋白质芯片技术筛选及确定白血病血清标志蛋白质[J]. 基础医学与临床, 2007, 27(3): 333-336.

. Screening of serum biomarkers in leukemia bu SELDI technique:a preliminary study[J]. Basic & Clinical Medicine, 2007, 27(3): 333-336.