基础医学与临床 ›› 2007, Vol. 27 ›› Issue (12): 1347-1351.

• 研究论文 • 上一篇    下一篇

牛磺酸对糖尿病大鼠坐骨神经雪旺细胞凋亡的影响

叶仁群 陈泽奇 林国彬 李玉红 贺钰磊 凌江红 申定珠   

  1. 深圳市宝安区中医院内科 中南大学湘雅医院中西医结研所 深圳市宝安区中医院内科 中南大学湘雅医院中西医结研所 中南大学湘雅医院中西医结研所 广西医科大学一附院中医科 中南大学湘雅医院中西医结研所
  • 收稿日期:2006-12-12 修回日期:2007-05-29 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 叶仁群

Taurine inhibited Schwann cell apoptosis of sciatic nerve in diabetic rat

Ren-qun YE, Ze-qi CHEN, Guo-bin LIN, Yu-hong LI, Yu-lei HE, Jiang-hong LING, Ding-zhu SHEN   

  1. Traditional Chinese medical hospital in Bao'an district of Shenzhen city
  • Received:2006-12-12 Revised:2007-05-29 Online:2007-12-25 Published:2007-12-25
  • Contact: Ren-qun YE,

摘要: 目的 观察牛磺酸对糖尿病大鼠坐骨神经雪旺细胞凋亡的影响,探讨其神经保护作用机制。方法 用链脲佐菌素(STZ)诱发糖尿病模型。牛磺酸治疗8W后应用化学比色法测定坐骨神经组织中丙二醛(MDA)和谷胱甘肽(GSH)含量;用免疫组化法、RT-PCR和TUNEL法检测坐骨神经中活化的caspase-3蛋白和mRNA表达,以及雪旺细胞凋亡的情况。结果 牛磺酸能降低坐骨神经中MDA水平,提高GSH水平(均P<0.05)。在正常鼠坐骨神经中,未见活化的caspase-3蛋白表达和细胞凋亡; caspase-3 mRNA有极少量表达。模型对照组上述表达均增加,牛磺酸干预后其表达均较模型组表达明显减少(P<0.05)。结论 牛磺酸抑制caspase-3 mRNA的转录与caspase-3的活化,减少细胞凋亡,与调节机体内的氧化应激状态有关,从而达到神经保护作用。

Abstract: Objective To observe the effects of Taurine on Schwann cell apoptosis of sciatic nerve in diabetic rat , and explore its mechanism of nervous protective effect。Methods: the diabetic models were induced by streptozotocin . the sciatic nerve were taken out in 8th week from the beginning of treatment, and then various parameters were measured as follows: The content of MDA and GSH in sciatic nerve were analysed by chromatometry ;caspase-3 expression in protein and mRNA level in sciatic nerve were analysed by immunohistochemical and reverse-transcriptase polymerase chain reaction(RT-PCR) method; Apoptosis of Schwann cell by method of TUNEL.Results: Taurine-treated control group could attenuate the content of MDA in sciatic nerve, and elevate the content of GSH(p<0.05).We did not find the Protein expression of actived caspase-3 and Apoptosis of Schwann cell in sciatic nerve of normal control group, but the caspase-3mRNA expression were at lower level in normal group than in model group(P<0.05).Taurine group significantly decreased the expression of caspase-3 and Apoptosis of Schwann cell (P<0.05).Conclusion: Taurine inhibiting transcription of caspase-3mRNA and activation of caspase-3 and decreasing apoptosis may be through adjusting the oxidative stress state of organism . This may be one of the function mechanisms of protective effects of Taurine on diabetic peripheral neuropathy.