曲古抑菌素A促进大鼠骨髓间充质干细胞向心肌样细胞分化

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 704-708.

曲古抑菌素A促进大鼠骨髓间充质干细胞向心肌样细胞分化

赵莉莉朱静冯川杨舸田杰

重庆医科大学儿童医院重庆医科大学儿科研究所重庆医科大学儿童医院重庆医科大学儿科研究所重庆医科大学儿童医院

收稿日期:2008-12-25 修回日期:2009-03-26 出版日期:2009-07-20 发布日期:2009-07-20
通讯作者: 朱静

TSA Promotes Mesenchymal Stem Cells differentiating into cardiomyocyte-like cells

Li-li ZHAO, Jing ZHU, Chuan FENG, Ge YANG, Jie TIAN

Affiliated Children Hospital, Chongqing University of Medical Science Pediatrics Institute; Chongqing University of Medical Science

Received:2008-12-25 Revised:2009-03-26 Online:2009-07-20 Published:2009-07-20
Contact: Jing ZHU,

摘要/Abstract

摘要： 目的观察不同浓度曲古抑菌素A(TSA)对骨髓间充质干细胞(MSCs)向心肌样细胞分化的作用，寻求适宜有效的干预条件。方法不同浓度TSA对MSCs进行不同时间干预，测定去乙酰化酶活性；相差显微镜下观察其形态；荧光免疫组化鉴定心肌特征性肌钙蛋白T（cTnT）的表达；RT-PCR检测Nkx-2.5、GATA-4的表达。结果在300nmol/L干预24h时去乙酰化酶活性最低；cTnT及Nkx-2.5、GATA-4基因表达随干预浓度逐渐增强，在300nmol/L干预24h时表达均趋于稳定。结论 TSA在体外适宜的浓度下可促进MSCs向心肌样细胞分化。

关键词: 去乙酰化酶抑制剂, 骨髓间充质干细胞, 细胞分化, 心肌样细胞

Abstract: Objective To observe the effect of TSA to induce mesenchymal stem cells(MSCs) differentiating into cardiomyocyte cells, to explore the effective conditions． Methods MSCs were incubated in different concentration of TSA and different duration time, then detected the histone deacetylase(HDAC) activity; the differentiation of MSCs was observed by phase-contrast mictmcopy;fluorescence immunohistochemistry assessed cardiac troponin T and RT-PCR detected the expression of Nkx-2.5、GATA-4 genes．Result The HDAC activity was the lowest when incubated with 300nmol/L TSA for 24 hours, expression of cTnT of the cells was enhanced after incubated with TSA. The expression levels of Nkx-2.5、GATA-4 genes were enhanced before interference.Conclusion TSA can advance MSCs to differentiate into cardiomyocyte cells in vitro in a suitable condition.

Key words: histone deacetylase inhibitors, mesenchymal stem cells, diferentiation, cadiocyte

赵莉莉朱静冯川杨舸田杰. 曲古抑菌素A促进大鼠骨髓间充质干细胞向心肌样细胞分化[J]. 基础医学与临床, 2009, 29(7): 704-708.

Li-li ZHAO; Jing ZHU; Chuan FENG; Ge YANG; Jie TIAN. TSA Promotes Mesenchymal Stem Cells differentiating into cardiomyocyte-like cells[J]. Basic & Clinical Medicine, 2009, 29(7): 704-708.

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