关键词: miR-203a-3p, 转化生长因子-β1(TGF-β1), 肝细胞癌

Abstract: Objective To explore the molecular mechanism of regulation on the proliferation and apoptosis in hepatocellular carcinoma(HCC) cells by miR-203a-3p. Methods RT-qPCR was used to detect the expression of miR-203 a-3p in the sample of liver cancer tissues and liver cancer cell lines. The HepG2 cell model expressing miR-203a-3p was established. Cell proliferation was observed by MTT method and the apoptosis of cells was detected by flow cytometry. Plate colony formation assay was used to detect clone formation and cell scratch assay was used to detect cell migration. Expression profile chip was applied in the detection of mRNA and ELISA was used to detect the secretion of transforming growth factor-β1 (TGF-β1). TGF-β1 protein expression was examined by Western blod technology. Results miR-203a-3p was weekly expressed in HCC tissues (P<0.001)and was negatively correlated with tumor stage (P<0.05). Over-expression of miR-203a-3p in HepG2 cells significantly reduced cell proliferation (P<0.05), migration (P<0.05), clone formation (P<0.05) and promoted cell apoptosis(P<0.05); TGF-β1 expression decreased(P<0.05) while the expression of TGFβR1 increased (P<0.05); The content of TGF-β1 in the cell culture supernatant decreased (P<0.05). The protein of TGF-β1 in cells decreased (P<0.05) and TGF-β1 recombinant protein reversed the miR-203a-3p induced inhibition of proliferation as well as migration (P<0.05). Conclusions miR-203a-3p inhibits proliferation of human hepatocellular carcinoma cell lines, promotes apoptosis and inhibits the expression of TGF-β1 protein.

Key words: miR-203a-3p, transforning growth factor-β1(TGF-β1), hepatocellular carcinoma