关键词: miR-30b, 脆性组氨酸三联体, 弥漫大B细胞淋巴瘤, 细胞活性

Abstract: Objective To study the mechanism of miR-30b by regulating fragile histidine triad gene(FHIT) on cell activity diffuse large B-cell lymphoma(DLBCL). Methods The tumor paraffin specimens of DLBCL patients and the paraffin markers of lymph node hyperplasia at the same time were selected from the Shanghai Minhang District Tumor Hospital (February 2017-June 2019) and divided them into the DLBCL group and the control group. DLBCL cells were divided into the WW group (DLBCL non transfected group), WZ group (DLBCL transfected NC group )and ZR group (DLBCL transfected miR-30b group). RT-qPCR method was used to detect miR-30b expression; Western blot was used to detect FHIT protein expression; colorimetric method was used to detect caspase-3 activity; Flow cytometry was used to detect DLBCL cell apoptosi and Transwell method was applied to detect cell invasion. Results The expression of miR-30b and FHIT in the control group was significantly higher than those in the DLBCL group (P＜0.05); the expression of miR-30b, FHIT and caspase-3 in the ZR group was higher than those in the WW and WZ groups (P＜0.05) The apoptosis rate of ZR group was time-dependently higher than that of WW and WZ groups(P＜0.05); the counting of DLBCL cell invasion in ZR group was significantly less than that of WW and WZ groups (P＜0.05). Conclusions After miR-30b transfection, the apoptosis of diffuse large B-cell lymphoma cells increases and the counting of invasion cells decreases. The mechanism of this result may be related to the enhancement of miR-30b, FHIT, and caspase-3 level.

Key words: miR-30b, fragile histidine triad gene, diffuse large B-cell lymphoma, cell activity