真菌PH1008胞外多糖抗小鼠阴道白假丝酵母菌感染

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (2): 155-158.

• 研究论文 • 下一篇

真菌PH1008胞外多糖抗小鼠阴道白假丝酵母菌感染

王英¹, 贝宁³, 裴华^2*, 杨文¹, 王华民¹

1.海南医学院病原生物学与免疫学实验室, 海南海口 571199;
2.海南医学院第二附属医院检验科,海南海口 570000;
3.海南省老年病医院四科,海南海口 571100

收稿日期:2020-01-09 修回日期:2020-05-25 出版日期:2021-02-05 发布日期:2021-01-19
通讯作者: ^*phzmh61@sohu.com
基金资助:
国家自然科学基金(31260225)

The anti-Candida albicans infection of mouse by extracellular polysaccharide from PH1008

WANG Ying¹, BEI Ning³, PEI Hua^2*, YANG Wen¹, WANG Hua-min¹

1. Laboratory of Pathogenic Biology and Immunology, Hainan Medical University, Haikou 571199;
2. Department of Laboratory, the Second Affiliated Hospital,Hainan Medical University,Haikou 570000;
3. the Fourth Department of Hainan Geriatric Hospital,Haikou 571100,China

Received:2020-01-09 Revised:2020-05-25 Online:2021-02-05 Published:2021-01-19
Contact: ^*phzmh61@sohu.com

摘要/Abstract

摘要： 目的评估从红树林土壤中分离的真菌PH1008胞外多糖在小鼠阴道白假丝酵母菌感染模型中的抗感染效应,为抗假丝酵母菌感染开发新的治疗药物提供思路。方法用真菌PH1008胞外多糖干预小鼠阴道白假丝酵母菌感染模型,提取阴道组织进行转录组基因表达水平测定以评估胞外多糖的抗感染效应。结果增强了肝细胞生长因子、补体1受体等基因的表达水平,且这一上调现象与多个信号传导途径密切相关。结论真菌PH1008胞外多糖局部干预小鼠阴道后可诱导机体产生抗白假丝酵母菌感染效应,相关机制有待于进一步研究。

关键词: 红树林真菌, 感染, 阴道

Abstract: Objective To evaluate the anti-infective effect of polysaccharide isolated from fungus PH1008 in vaginal Candida albicans infection model of mouse,and to provide a new and potential pathway to the development of anti-candida drugs. Methods Determination of gene expression level of transcriptome in mice vagina after yeast infection mouse model was treated by exopolysaccharide of fungus PH1008. Results The expression of hepatocyte growth factor and complement 1 receptor was increased, and the up-regulation was related to varied signal transduction pathways. Conclusions The anti-infection effect of Candida albicans can be induced by vaginal mucosal immunization with exopolysaccharide of fungus PH1008, and the relevant mechanism needs further research.

Key words: mangrove fungus, infection, vagina

中图分类号:

王英, 贝宁, 裴华, 杨文, 王华民. 真菌PH1008胞外多糖抗小鼠阴道白假丝酵母菌感染[J]. 基础医学与临床, 2021, 41(2): 155-158.

WANG Ying, BEI Ning, PEI Hua, YANG Wen, WANG Hua-min. The anti-Candida albicans infection of mouse by extracellular polysaccharide from PH1008[J]. Basic & Clinical Medicine, 2021, 41(2): 155-158.

参考文献

[1] Sun MG, Huang Y, Xu YH, et al. Efficacy of vitamin B complex as an adjuvant therapy for the treatment of complicated vulvovaginal candidiasis: an in vivo and in vitro study[J]. Biomed Pharmacother, 2017, 88(Complete): 770-777.
[2] Zeng X, Zhang Y, Zhang T, et al. Risk factors of vulvovaginal candidiasis among women of reproductive age in Xi'an: a cross-sectional study[J]. Biomed Res Int, 2018, 2018:1-8.
[3] 孙明伟, 丁艳娇, 鲁春华, 等. 海南红树林根际糖多胞菌菌株YIMM135 68的红霉素类抗生素的分离和鉴定[J]. 中国抗生素杂志, 2017,42:346-350.
[4] 王永霞, 黄燕妮, 李国军, 等. 海南红树林淡紫拟青霉EPS纯化物体外抗HSV-1实验研究[J]. 海南医学院学报, 2016, 22:2217-2220.
[5] Yang L, Wu H, Qiu W, et al. Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the dectin-1 signaling pathway[J]. J Ethnopharmacol, 2018, 223:51-62.
[6] Zheng YF, Zhang Q, Liu XM, et al. Extraction of polysaccharides and its antitumor activity on magnolia kwangsiensis figlar & noot[J]. Carbohydr Polym, 2016, 142:98-104.
[7] Ilhem R, Anouar F, Anouar BS, et al. Lycium europaeum, linn as a source of polysaccharide with, in vitro, antioxidant activities and, in vivo, anti-inflammatory and hepato-nephroprotective potentials[J]. J Ethnopharmacol, 2018,8:116-127.
[8] 潘峰, 姚芸欣, 唐鑫, 等. 瓦布贝母内生真菌Fusarium redolens 6WBY3多糖的理化性质及抗氧化活性[J]. 微生物学报, 2017, 57:240-253.
[9] Guo Z, Gai C, Cai C, et al. Metabolites with insecticidal activity from asper-gillus fumigatus JRJ111048 isolated from mangrove plant acros-tichum specioum endemic to Hainan island[J]. Mar Drugs, 2017,15:381.
[10] Pavan KJ, Ajitha G, Gothandam K. Bioactivity assess-ment of Indian ori gin—mangrove actinobacteria against Candida albicans[J]. Mar Drugs, 2018, 16:60.
[11] Rahman M,Segal BH. Thematic issue: immunity, inflammation and fungal infections[J]. Immunological Investigations, 2001,40:670-675.
[12] Verma A, Wuthrich M, Deepe G, et al. Adaptive immunity to fungi[J]. Cold Spring Harb Perspect Med, 2015, 5.doi:10.1101/cshperspect.a019612.

[1]	朱李茹, 黄友明, 章宏祥, 周发友, 唐晓磊. 葡聚糖结合蛋白的原核表达及检测深部真菌感染的应用[J]. 基础医学与临床, 2022, 42(6): 883-889.
[2]	杜心怡, 毛小荣, 李俊峰. 抗-HBc定量测定在乙型肝炎病毒慢性感染性疾病中的应用[J]. 基础医学与临床, 2022, 42(5): 804-808.
[3]	钟逸锋宋英娜汤萍萍尹婕. 手把手传授与录像演示在胎头吸引器助产教学中的效果评估[J]. 基础医学与临床, 2021, 41(6): 918-922.
[4]	夏迪, 许力, 李旭, 黄宇光. 目标导向液体治疗优化老年腰椎手术患者术中血流动力学降低术后并发症[J]. 基础医学与临床, 2021, 41(2): 178-183.
[5]	冯晓凯何怀武张佳慧程卫隆云郭伏平. 罕见酵母菌-胶红酵母菌血流感染1例[J]. 基础医学与临床, 2021, 41(11): 1658-1661.
[6]	许惠娟刘坤郑景妹姜秋利鲁华东许建芳. 厦门地区3 144例女性人乳头瘤病毒（HPV）感染及宫颈病变筛查分析[J]. 基础医学与临床, 2021, 41(11): 1643-1647.
[7]	陈娟, 史宏晖, 张林, 张国瑞, 王乃利, 朱兰. 基于新鲜尸体的结构化培训在经阴道植入网片盆底重建手术教学中的应用[J]. 基础医学与临床, 2020, 40(9): 1269-1272.
[8]	胡晓焱杨琼秀肖春桥全丽丽陈冬云. 新型冠状病毒肺炎疫情期间定点医院缓冲隔离病区的建立与运行[J]. 基础医学与临床, 2020, 40(6): 746-749.
[9]	佟乐陈晓莉叶磊. 新型冠状病毒肺炎武汉一线支援队华西医疗队驻地管理[J]. 基础医学与临床, 2020, 40(5): 598-600.
[10]	王敏刘鑫. 布鲁氏菌感染的固有免疫机制研究进展[J]. 基础医学与临床, 2018, 38(9): 1352-1355.
[11]	牛书俐庞骁周莉李素平. RGD三肽的临床应用进展[J]. 基础医学与临床, 2018, 38(1): 107-111.
[12]	申丽红陈永德朝浩鹏宋文琪田晓怡. 中性粒细胞/淋巴细胞比值对血流感染的诊断价值[J]. 基础医学与临床, 2017, 37(7): 1000-1003.
[13]	赵群陈志恒朱小玲李志跃. 湖南省24 817例体检妇女筛查宫颈病变结果分析[J]. 基础医学与临床, 2015, 35(4): 435-438.
[14]	李霖琳谢正德刘春艳相子春任丽丽申昆玲王健伟. 北京地区儿童急性上呼吸道感染中肠道病毒感染谱的分析[J]. 基础医学与临床, 2014, 34(4): 485-489.
[15]	赵慧王亮马秀敏丁剑冰. Tim-3及其配体Galectin-9与病毒感染的关系[J]. 基础医学与临床, 2012, 32(9): 1095-1098.

真菌PH1008胞外多糖抗小鼠阴道白假丝酵母菌感染

The anti-Candida albicans infection of mouse by extracellular polysaccharide from PH1008

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价