黄芪主要成分对TGF-β1诱导大鼠睾丸Leydig细胞系合成雌二醇的影响

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (1): 68-71.

黄芪主要成分对TGF-β1诱导大鼠睾丸Leydig细胞系合成雌二醇的影响

李彦荣^1*, 刘光炜¹, 赵晶², 李亚军¹

1.甘肃中医药大学(定西校区),甘肃定西 743000;
2.定西市人民医院检验科,甘肃定西 743000

收稿日期:2019-04-06 修回日期:2020-09-30 出版日期:2021-01-05 发布日期:2020-12-30
通讯作者: ^*38746648@qq.com
基金资助:
2018年度甘肃省高等学校科研项目(2018A-180);甘肃中医药大学定西校区项目(2017XJZD09)

Effects of main compositions of Astragalus on estradiol synthesizing activing in TGF-β1-induced rat testicular Leydig cell line

LI Yan-rong^1*, LIU Guang-wei¹, ZHAO Jing², LI Ya-jun¹

1. Gansu University of Traditional Chinese Medicine(Dingxi Campus), Dingxi 743000;
2. Department of Clinical Laboratory, Dingxi People's Hospital, Dingxi 743000, China

Received:2019-04-06 Revised:2020-09-30 Online:2021-01-05 Published:2020-12-30
Contact: ^*38746648@qq.com

摘要/Abstract

摘要： 目的探讨黄芪主要成分对TGF-β1诱导下大鼠睾丸Leydig细胞及该细胞合成雌二醇(E₂)、P450芳香化酶(P450arom)的影响。方法将体外培养的Leydig细胞分为对照组、模型组(5 ng/mL TGF-β1造模)和中药干预3组,在造模后分别用终浓度为10和50 mg/L黄芪总黄酮、终浓度为10和50 mg/L黄芪总多糖、终浓度20和100 mg/L的黄芪总皂苷干预。48 h后进行细胞爬片,用ELISA检测细胞中E₂、P450arom蛋白含量;Imagej-win64软件计数细胞;用SPSS 20.0、GraphPad Prism 6.0对数据进行统计分析。结果模型组Leydig细胞和对照组相比,形态异常,数量减少(P＜0.01);明显抑制Leydig细胞合成E₂和P450arom(P＜0.05)。黄芪总黄酮、黄芪总皂苷和黄芪总多糖均能够改善TGF-β1诱导下Leydig细胞病变,细胞数量明显增多(P＜0.01),三者均能缓解TGF-β1抑制细胞合成E₂的作用,20 mg/L黄芪总皂苷的效果最显著。结论黄芪总皂苷是黄芪中减轻TGF-β1抑制睾丸Leydig细胞合成E₂作用效果最佳的成分。

关键词: 黄芪, 睾丸Leydig细胞, 雌二醇(E₂)

Abstract: Objective To investigate the effects of the main compositions of Astragalus on testicular Leydig cells and their synthesis of estradiol(E₂), P450arom under TGF-β1 induction. Methods Leydig cells were divided into three groups: control group, model group and Chinese herbal medicine intervention group. Results Compared with the control group, Leydig cells in the model group showed abnormal morphology and less cells(P＜0.01).TGF-β1 significantly inhibited the synthesis of E₂ and P450arom by Leydig cells (P＜0.05). Total Astragalus flavone, total Astragalus saponin and total astragalus polysaccharide all resulted in pathological changes of Leydig cells induced by TGF-β1. Compared with the model group, the number of cells increased significantly (P＜0.01) and all of them alleviated the inhibitory effect of TGF-β 1 on the synthesis of E₂ in Leydig cells induced by TGF-β1, and the effect of concentration of 20 mg/L total saponin of Astragalus was the most significant. Conclusions Total Astragalus saponin is the most effective component in Astragalus to exert TGF-β1 inhibitory effect on E₂ synthesis by Leydig cells.

Key words: Astragalus, Leydig cells of testis, estradiol(E₂)

中图分类号:

R285.5

李彦荣, 刘光炜, 赵晶, 李亚军. 黄芪主要成分对TGF-β1诱导大鼠睾丸Leydig细胞系合成雌二醇的影响[J]. 基础医学与临床, 2021, 41(1): 68-71.

LI Yan-rong, LIU Guang-wei, ZHAO Jing, LI Ya-jun. Effects of main compositions of Astragalus on estradiol synthesizing activing in TGF-β1-induced rat testicular Leydig cell line[J]. Basic & Clinical Medicine, 2021, 41(1): 68-71.

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