人胚胎干细胞RNA的细胞质和细胞核内的定位

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (6): 765-770.

人胚胎干细胞RNA的细胞质和细胞核内的定位

周凡琦¹, 冯文新², 谭普文³, 马艳妮¹, 王栋³, 余佳^1*

1.中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室, 北京 100005;
2.河南地矿职业学院, 河南郑州 450000;
3.南方医科大学基础学院生物信息系, 广东广州 510000

收稿日期:2020-03-08 修回日期:2020-04-19 出版日期:2020-06-05 发布日期:2020-05-29
通讯作者: *j-yu@ibms.pumc.edu.cn
基金资助:
国家自然科学基金重点项目(81530007)

RNA localization in cytoplasm and nucleus of human embryonic stem cells

ZHOU Fan-qi¹, FENG Wen-xin², TAN Pu-wen³, MA Yan-ni¹, WANG Dong³, YU Jia^1*

1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. Henan Geology Vocational College, Zhengzhou 450000;
3. Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510000, China

Received:2020-03-08 Revised:2020-04-19 Online:2020-06-05 Published:2020-05-29
Contact: *j-yu@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的探讨人胚胎干细胞(hESCs)中RNA细胞核和细胞质内分布信息。方法分离hESCs细胞质与细胞核后提取RNA并测序,生物信息学分析核和质中RNA定位特点,并使用RT-qPCR实验技术验证RNA的定位。结果 hESCs细胞质中RNA种类及其特异定位的RNA均多于细胞核,其中特异性定位在细胞质中的RNA有2 952个,细胞核中634个。结论描绘hESCs中RNA细胞核和细胞质定位图谱,鉴定了多个特异定位在细胞核与细胞质的RNA。

关键词: 人胚胎干细胞, RNA定位

Abstract: Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified.

Key words: human embryonic stem cells (hESCs), RNA localization

中图分类号:

Q522

周凡琦, 冯文新, 谭普文, 马艳妮, 王栋, 余佳. 人胚胎干细胞RNA的细胞质和细胞核内的定位[J]. 基础医学与临床, 2020, 40(6): 765-770.

ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia. RNA localization in cytoplasm and nucleus of human embryonic stem cells[J]. Basic & Clinical Medicine, 2020, 40(6): 765-770.

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