IL-8在胃癌组织上皮间质转化过程中表达增强

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (10): 1444-1448.

IL-8在胃癌组织上皮间质转化过程中表达增强

方复,毛华辉,卢国春,郭渊,童向美

桐庐县第一人民医院

收稿日期:2018-08-15 修回日期:2019-02-11 出版日期:2019-10-05 发布日期:2019-09-25
通讯作者: 方复 E-mail:fangfutl@163.com
基金资助:
杭州市科技计划引导项目（农业与社会发展）

Enhanced IL-8 expression in epithelial mesenchymal transformation of gastric cancer tissue

Received:2018-08-15 Revised:2019-02-11 Online:2019-10-05 Published:2019-09-25

摘要/Abstract

摘要： 目的研究胃癌上皮间质转化（EMT）过程中趋化因子IL-8的调节机制与意义。方法用Western blot、酶联免疫吸附试验（ELISA）等技术，检测胃癌组织与正常组织中IL-8以及EMT相关基因（E-cadherin, vimentin, twist等）的表达，胃癌细胞系MGC-803对IL-8与胃癌细胞EMT的相关性进行分析和探讨。结果肿瘤组织中IL-8的表达水平（710.5±89.9）显著高于癌旁组织（510.5±85.6)（P<0.001）。与正常组织相比，胃癌组织中 IL-6、twist 及 vimentin 表达水平明显高于正常胃黏膜组织（P<0.05），而E-cadherin表达水平低于正常组织（P<0.05）。IL-8因子的分泌与twist蛋白（P<0.001），和vimentin蛋白（r＝0.454，P<0.001）表达水平呈显著正相关性，与E-cadherin蛋白表达水平呈显著负相关性（P<0.001）。结论胃癌组织中趋化因子IL-8的表达量增强， IL-8与胃癌侵袭转移相关，同时IL-8的表达量也可作为胃癌侵袭转移病人预后判断的参考标准之一。

关键词: 胃癌, IL-8, 上皮间质转化, 侵袭和转移

Abstract: Objective To investigate the regulatory mechanism and significance of chemokine IL-8 in epithelial mesenchymal transition (EMT) leading to gastric cancer. Methods Western blot, enzyme-linked immunosorbent assay (ELISA) and other techniques were used to detect the expression levels of IL-8 and EMT-related genes (E-cadherin, vimentin, twist, etc.) in gastric cancer tissues and plasma. To study the correlation between IL-8 and EMT in gastric cancer cells with MGC-803. Results The expression level of IL-8 in tumor tissues (710.53±89.92) was significantly higher than that in adjacent tissues (510.52±85.45), and the difference was statistically significant (P<0.001). Compared to normal tissue，the expression rates of IL-8, Twist and vimentin in gastric cancer tissues were significantly higher than those in normal gastric mucosa tissues（P<0.05）, while the expression rates of E-cadherin in gastric cancer tissues were lower than those in normal tissues（P<0.05).The secretion of IL-8 was positively correlated with Twist protein (P < 0.001), vimentin protein (r=0.454, P< 0.001), and negatively correlated with E-cadherin protein (P< 0.001). Conclusion The expression of chemokine IL-8 is increased in gastric cancer tissues, and its expression level is correlated with the expression of EMT-related proteins, which indicates that IL-8 is related to the invasion and metastasis of gastric cancer. Meanwhile, the expression of IL-8 may also be used as one of the criteria in the prognosis of patients, for the invasion and metastasis of gastric cancer.

Key words: gastric cancer, IL-8, epithelial-mesenchymal transition, invasion and metastasis

方复毛华辉卢国春郭渊童向美. IL-8在胃癌组织上皮间质转化过程中表达增强[J]. 基础医学与临床, 2019, 39(10): 1444-1448.

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