关键词: CKS2, 卵巢癌, 细胞生长, 选择性剪接

Abstract: Objective To construct the lentivirus expression vector of shRNA targeting CKS2 gene and explore the effects of CKS2 knockdown on A2780 cell proliferation. Methods Two oligonucleotides targeting CKS2 gene and their negative control shRNA (NC shRNA) were synthesized and cloned into lentivirus expression vector PLKO.1. The plasmids PLKO.1/CKS2 shRNA1, PLKO.1/CKS2 shRNA2 and PLKO.1/NC shRNA were transfected into 293T cells and lentivirus were prepared. CKS2 expression levels were determined by real time PCR and Western blot in A2780 cells infected with lentivirus. The effects on A2780 cell proliferation were determined by MTT; PARP-1 cleavage was examined by Western blot analysis; apoptosis was determined by flow cytometry. Results The lentivirus expression vectors containing CKS2 shRNA were constructed successfully, and the lentivirus were produced. The CKS2 expression levels were decreased obviously after infection by lentivirus（P < 0.05）; CKS2 knockdown could inhibit A2780 cell proliferation（P < 0.05）, and induce PARP-1 cleavage and apoptosis（P < 0.05）. Conclusions The lentivirus expression vectors containing CKS2 shRNA were constructed successfully, and could inhibit A2780 cell proliferation.

Key words: CKS2, Ovarian cancer, Cell growth, Alternative splicing