血管细胞黏附分子-1蛋白在人脑胶质瘤组织内表达改变及其临床意义

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (12): 1600-1604.

血管细胞黏附分子-1蛋白在人脑胶质瘤组织内表达改变及其临床意义

郭颖¹,曲彦明²,韩松²,钟洁¹,于春江³,李俊发¹

1. 首都医科大学
2. 首都医科大学神经生物学系
3. 首都医科大学第十一临床医学院，北京三博脑科医院

收稿日期:2013-08-21 修回日期:2013-09-20 出版日期:2013-12-05 发布日期:2013-11-28
通讯作者: 李俊发 E-mail:junfali@ccmu.edu.cn
基金资助:
首都医科大学基础临床合作研究基金

Changes of VCAM-1 protein expression in human brain glioma and its clinical significance

Received:2013-08-21 Revised:2013-09-20 Online:2013-12-05 Published:2013-11-28
Contact: Jun-fa LI E-mail:junfali@ccmu.edu.cn

摘要/Abstract

摘要： 目的观察不同级别人脑胶质瘤组织中VCAM-1的表达情况，探讨其与脑胶质瘤病理分级的关系及临床意义。方法用免疫组织化学及蛋白免疫印迹技术，检测6例正常脑组织和30例脑胶质瘤组织中VCAM-1蛋白表达情况，比较各级别之间的表达差异，分析VCAM-1蛋白表达水平与脑胶质瘤病理级别的相关性。结果正常脑组织及各级别胶质瘤组织内VCAM-1阳性细胞率分别为：正常5.8%，II级26.5%，III级69.5%和IV级79.3%。高级别脑胶质瘤组织内，阳性细胞集中分布于肿瘤微血管周围。脑胶质瘤组织阳性细胞率明显多于正常脑组织(p<0.05)；III级和IV级恶性胶质瘤阳性细胞率显著高于II级低级别胶质瘤 (p<0.05)；正常脑组织中VCAM-1表达量较低，胶质瘤组织中表达量则明显增多(p<0.05)；III级和IV级恶性胶质瘤内VCAM-1蛋白表达明显高于II级胶质瘤(p<0.05)；而III级和IV级脑胶质瘤间，VCAM-1蛋白表达则无显著性差异。结论 VCAM-1蛋白在人脑胶质瘤组织中明显高表达，且与脑肿瘤病理级别相关，提示VCAM-1蛋白在脑胶质瘤恶性进展中可能发挥着重要作用。

关键词: 血管细胞黏附分子-1, 蛋白表达和分布, 脑胶质瘤, 肿瘤病理分级, 临床意义

Abstract: Objective By observing the changes of vascular cell adhesion molecule-1 (VCAM-1) protein expression in human brain glioma tissue to determine its correlation with tumor pathological grade and clinical significance. Methods The distribution and changes of VCAM-1 protein expression in 6 normal brain and 30 brain glioma tissue were detected by using immunohistochemistry staining and Western blot analysis. Results The results of immunohistochemistry staining showed that the percentage of VCAM-1 positive cell number in normal brain tissue, grade II, III and IV brain glioma tissue were 5.8%, 26.5%, 69.5% and 79.3%, respectively. The VCAM-1 positive cells mainly distributed along the tumor capillaries in high pathological grade glioma. The number of VCAM-1 positive cells in glioma tissue was significantly higher than that of normal brain tissue (p<0.05). The higher number of VCAM-1 positive cells in grade III and IV glioma tissue was observed when compared with that of grade II glioma tissue(p<0.05), but no significant difference was found between grade III and grade IV glioma tissue (p=0.068). Similarly, the Western blot analysis alse indicated that there were more VCAM-1 protein expressions in brain glioma tissue with the increase of the tumor grade level when compared with the less VCAM-1 expressed in normal brain tissue (p<0.05). In addition ,the VCAM-1 protein expressions in high pathological grade III and IV glioma tissue extensively increased compared with glioma II glioma tissue (p<0.05), but no significant difference in VCAM-1 protein expressions was observed between grade III and IV glioma tissue. Conclusion The significant increase of VCAM-1 protein expressions in brain glioma tissue positively correlated with the tumor pathological grades, suggesting an important role of VCAM-1 in the malignant progress of human brain glioma.

Key words: vascular cell adhesion molecule-1(VCAM-1), protein expression and distribution, brain glioma, tumor pathological grade, clinical significance

中图分类号:

R739.42

郭颖曲彦明韩松钟洁于春江李俊发. 血管细胞黏附分子-1蛋白在人脑胶质瘤组织内表达改变及其临床意义[J]. 基础医学与临床, 2013, 33(12): 1600-1604.