关键词: ESTs, 生物信息学分析, long nonconding RNA, 筛选, 鉴定, 表达谱分析

Abstract: Objective Screening and identification of long noncoding RNAs in PCBP2 gene locus based on ESTs. Methods Screening ESTs with introns transcription, with the analysis of CPC and statistical comparison, four ESTs were recognized as potential lncRNAs（two of the four are human ESTs, the other two are mouse ESTs）, those ESTs were identified with polymerase chain reaction( PCR ) and then cloned in pGEM-T Vector-System for sequencing. We test the express of these ESTs in human cell lines or mouse tissue. Results The four ESTs are potential long noncoding RNAs, three out of four ESTs were identified, their expression were different. Conclusions With a new method for searching lncRNAs based on ESTs, we recognized some potential long noncoding RNAs, one of which may be a functional lncRNA influence mouse brain development.

Key words: ESTs, bioinformatic analysis, long noncoding RNA, screening, identification, expression