关键词: TP53TG1, U87MG细胞, GRP78, IDH 1, PKM2

Abstract: Objective To construct full length clone of TP53TG1 into eukaryotic expression vector, and to explore its overexpression effect on glucose deprivation stress response. Methods TP53TG1 was amplified from human glioma cell by RT-PCR, and the eukaryotic expression clone of TP53TG1 was constructed. Then, its expression in U87MG cells was detected by real time PCR. Furthermore, we overexpressed TP53TG1 and meanwhile treated with low glucose (0.3g/L, 8h) in U87MG cells, and measured the expression of GRP78, IDH1 and PKM2 mRNA by real time PCR. Results TP53TG1 eukaryotic expression clone was successfully constructed. After the clone was transfected into U87MG cells for 36 hours, green fluorescence was seen. The expression of TP53TG1 was increased by 2.9x106 times in U87MG cells (P<0.05). As a result of over expression of TP53TG1 and low glucose treatment simultaneously in U87MG cells, GRP78 and IDH1 mRNA expression were significantly increased (P<0.05), while PKM2 mRNA significantly reduced (P<0.05). Conclusion Results suggest that TP53TG1 may be involved in the stress response of U87MG cells under glucose deprivation through influencing the expression of GRP78, IDH1 and PKM2 mRNA.

Key words: TP53TG1, U87MG cells, GRP78, IDH 1, PKM2