基础医学与临床 ›› 2012, Vol. 32 ›› Issue (8): 884-888.

• 研究论文 • 上一篇    下一篇

基质细胞衍生因子-1α预处理对大鼠骨髓间充质干细胞体外存活及旁分泌的影响

束波1,刘志江2,范芳3   

  1. 1. 遵义医学院
    2. 遵义医学院第一附属医院
    3. 遵义医学院生物化学教研室
  • 收稿日期:2012-04-05 修回日期:2012-06-05 出版日期:2012-08-05 发布日期:2012-07-17
  • 通讯作者: 束波 E-mail:shubo1014@126.com
  • 基金资助:
    贵州省科学技术基金

Effects of SDF-1α preconditioning on survival and paracrine of rat marrow mesenchymal stem cells in vitro

  • Received:2012-04-05 Revised:2012-06-05 Online:2012-08-05 Published:2012-07-17

摘要: 摘 要: 目的 探讨基质细胞衍生因子-1α(SDF-1α)预处理对过氧化氢(H2O2)诱导的大鼠骨髓间充质干细胞(MSCs)损伤的保护作用及对其旁分泌的影响。方法 体外培养MSCs随机分为:正常对照组、过氧化氢损伤组、0.02 μg/ml SDF-1α预处理损伤组及0. 2 μg/ml SDF-1α预处理损伤组。观察SDF-1α对过氧化氢体外模拟缺血缺氧损伤后细胞的存活、凋亡、增殖及旁分泌的影响。结果 SDF-1α预处理MSCs后, 与损伤对照组相比,细胞释放LDH及细胞凋亡率明显降低,细胞增殖明显增多,血管内皮生长因子(VEGF)及碱性成纤维生长因子(bFGF)在细胞中的蛋白表达及细胞上清液中的活性明显增加。结论 SDF-1α预处理MSCs能有效减少细胞的损伤及凋亡,促进细胞的存活,增强细胞的旁分泌效应

关键词: 基质细胞衍生因子-1α, 预处理, 骨髓间充质干细胞, 旁分泌

Abstract: Abstract: Objective: To investigate effects of SDF-1α preconditioning on survival and paracrine of H2O2 treated rat marrow mesenchymal stem cells(MSCs). Methods: MSCs were randomly divided into the control group, H2O2 group, 0.02μg/ml SDF-1α +H2O2 precondition group, 0.2 μg/ml SDF-1α+H2O2 group. LDH were determined after different treatment. MSCs stained with Hoechst33342 for counting apoptosis percentage. Cells proliferation were detected by MTT. ELISA for detecting VEGF and bFGF. Expressions of VEGF and bFGF in MSCs detected by Western blot. Results SDF-1α preconditioning reduced LDH release and cell apoptosis, and increased cell proliferation. The protein levels of VEGF and bFGF in preconditioning group were significant higher than H2O2 group. Secretion of VEGF and bFGF from preconditioned MSCs were increased when compared with H2O2 group (P<0.05). Conclusions: SDF-1α preconditioning reduces cell damage and apoptosis, promotes cell survival and enhances the effects of paracrine.

Key words: SDF-1, precondition, marrow mesenchymal stem cells, paracrine

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