基础医学与临床 ›› 2012, Vol. 32 ›› Issue (7): 823-827.

• 研究论文 • 上一篇    下一篇

2,4-二甲基反式芪改善侧脑室注射A?25-35诱导的高脂大鼠学习记忆功能

谢沛希1,孙兰2,阮灿军2,李娜1,李展3   

  1. 1. 北京联合大学师范学院
    2. 中国医学科学院基础医学研究所
    3. 中国医学科学院药用植物研究所
  • 收稿日期:2011-10-13 修回日期:2012-02-20 出版日期:2012-07-05 发布日期:2012-06-20
  • 通讯作者: 孙兰 E-mail:762448405@QQ.com

Tran-2,4-dimethoxystibene (S3) promoted the learning and memory in model rats induced by hypercholesterolemic with i.c.v. injection of Aβ25-35

  • Received:2011-10-13 Revised:2012-02-20 Online:2012-07-05 Published:2012-06-20
  • Contact: Lan SUN E-mail:762448405@QQ.com

摘要: 摘要 目的 研究2,4-二甲基反式芪(S3)对侧脑室注射Aβ25-35引起的高脂大鼠学习记忆功能障碍的作用及初步机制。方法 雌性Wistar大鼠70只,分为正常对照组;单纯高脂组;高脂+脑室注射组;阳性药E2组(1mg/kg/d);S3高剂量组(50mg/kg/d);S3中剂量组(25mg/kg/d)及S3低剂量组(12.5 mg/kg/d)。除正常对照组10只大鼠外,其他组大鼠给予高脂饮食6周,测定血脂,然后给予侧脑室注射Aβ25-35,同时连续给药7天,从第三天起行Morris水迷宫及跳台实验,每天一次,连续4次,测定各组大鼠学习记忆功能;以分光光度法测定ChAT和AchE, ELISA法测定Ach浓度。结果 S3能够剂量依赖的降低侧脑室注射高脂大鼠血浆总胆固醇和LDL-C浓度(P<0.01)。高剂量组S3能够明显缩短找到平台时间、延长潜伏期并减少错误次数。同时,S3能增加海马组织ChAT活性和Ach浓度,降低AchE活性。结论 S3可能通过降低血脂浓度、增加ChAT活性、降低AchE活性而增加海马Ach浓度,从而改善模型大鼠的学习记忆功能。

关键词: 关键词:2,4-二甲基反式芪(S3), 高脂大鼠, 学习记忆, 胆碱, Aβ25-35

Abstract: Abstract Objective To investigate the effect of S3 on learning and memory in model rats induced by hypercholesterolemic with i.c.v. injection of Aβ25-35. Method 70 female Wistar rats were divided into 7 groups. Except the normal group, other 60 rats were fed with hypercholemic chow for six weeks, and then received an intracerebroventricular injection for once. The E2 and S3-treatment groups’ rats were treated with E2 or S3 for another 7 days. Behavioral changes were evaluated by Morris water maze and step-down test. The activities of choline acetyl transferase (ChAT) and acetylcholine esterase (Ach E) were analyzed by spectrophotometric method, and the content of acetylcholine was by ELISA. Result The data shown that S3 dose-dependently decreased serum total and LDL-C levels (P<0.01) in model rats with hypercholesterolemic plus i.v.c. injection of Aβ25-35. The highest dose of S3 shortened the escape latency significantly. The step-down latency of S3 treated groups was restored to near that of the control group and the number of errors was reduced markedly. Meanwhile, S3 reversed the decreased activity of ChAT as well as the increased activity of Ach E in hippocampus. Conclusion These findings suggest that S3 improved the model rats learning and memory by decreased the serum cholesterol, increased the concentration of Ach in hippocampus through changes of ChAT and Ach E activities.

Key words: Key Words: tran-2,4-dimethoxystibene (S3), hypercholesterolemic rat, learning and memory, cholinergic, Aβ25-35

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