基础医学与临床 ›› 2012, Vol. 32 ›› Issue (7): 739-743.

• 研究论文 • 上一篇    下一篇

大鼠脊髓硫酸软骨素蛋白多糖4细胞的生后发育特征

伍修宇1,孙善全2   

  1. 1. 重庆医科大学解剖学教研室
    2. 重庆医科大学神经科学研究中心
  • 收稿日期:2011-11-16 修回日期:2012-02-29 出版日期:2012-07-05 发布日期:2012-06-20
  • 通讯作者: 孙善全 E-mail:sunsq2151@sohu.com
  • 基金资助:
    骨髓间充质干细胞瘤化中STAT3信号通路过度激活的作用机制与规避研究;重庆市自然科学基金

Postnatal development characteristics of NG2 cells in spinal cords of rats

  • Received:2011-11-16 Revised:2012-02-29 Online:2012-07-05 Published:2012-06-20
  • Supported by:
    ;Natural Science Foundation Project of CQ CSTC

摘要: 目的 研究大鼠脊髓中硫酸软骨素蛋白多糖4(NG2)细胞生后发育特征。方法 采用免疫组织化学及Western blot,观察生后不同发育阶段的脊髓中NG2细胞形态变化。结果 免疫组织化学染色显示,在大鼠生后各发育阶段的脊髓内均有NG2细胞表达;脊髓内NG2细胞胞体逐渐增大,其形态由圆形或卵圆形变为不规则形,突起数目和长度随之增加。与同时期大脑皮层的NG2细胞形态比较,脊髓内的NG2细胞胞体要大,突起短而少。Western blot显示,P1d,脊髓内NG2表达水平最高, P7d开始下降,P21d最低,P60d回升。结论 在大鼠不同发育阶段的脊髓内,NG2细胞形态数量不同, P7d到P21d是脊髓内神经纤维髓鞘主要形成期;脊髓内NG2细胞可能处于少突胶质细胞系发育的早期阶段。

关键词: 硫酸软骨素蛋白多糖4, 脊髓, 发育, 大鼠

Abstract: Objective To observe the postnatal development characteristics of NG2 cells in spinal cord of rats. Method Immunohistochemistry(IHC) was applied to examine the morphologic characteristics of NG2 cells. Western blot was used to observed the expression of NG2 in spinal cord during the different postnatal developmental stages. Result IHC showed NG2 cells persistently existed during different postnatal developmental stages in spinal cord. There were a great number of NG2 cells with few processes and round or oval cell bodies at P1d. At P7d, a few NG2 cells can be detected with short processes in spinal cord. But at P21d,few NG2 cells were examined in the spinal cord. At P60d,NG2 cells with irregular cell bodies and 1~3 short processes were revealed. Compared with the cerebral cortex in the corresponding stages,NG2 cells in spinal cord possessed bigger soma, shorter and thin processes.Western blot showed that NG2 expression was highest at P1d ,declined at P7d and reduced significantly lowest at P21d,but increased atP60d.Conclusion The NG2 cells persistently exist in spinal cord and are different in cell number and morphology at the different postnatal development stages. It may start myelination from P7d to P21d.NG2 cells in spinal cord may be at an earlier stage in oligodendrocyte lineage.

Key words: NG2, spinal cord, development, rat

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