人脂肪来源的间充质干细胞诱导人乳腺癌MCF7细胞上皮间质转化

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (6): 623-627.

人脂肪来源的间充质干细胞诱导人乳腺癌MCF7细胞上皮间质转化

徐琦璘¹,赵春华²,王椋³

1. 北京协和医学院中国医学科学院基础所组织工程研究中心
2. 中国医学科学院基础医学研究所组织工程中心
3. 中国医学科学院基础医学研究所北京协和医学院组织工程中心

收稿日期:2012-03-07 修回日期:2012-04-25 出版日期:2012-06-05 发布日期:2012-05-25
通讯作者: 赵春华 E-mail:cb84@163.com; chunhuaz@public.tpt.tj.cn

Mesenchymal stem cells stimulate epithelial to mesenchymal transition in breast cancer cell line MCF7

Qi-Lin XU¹,Chun-hua ZHAOLiang WANG³

Received:2012-03-07 Revised:2012-04-25 Online:2012-06-05 Published:2012-05-25
Contact: Chun-hua ZHAO E-mail:cb84@163.com; chunhuaz@public.tpt.tj.cn

摘要/Abstract

摘要： 目的探讨人脂肪来源的间充质干细胞（haMSC）在乳腺肿瘤微环境中对乳腺癌细胞的影响。方法采用transwell体系间接共培养乳腺癌细胞系（MCF7）和人脂肪来源间充质干细胞，对共培养后的肿瘤细胞进行细胞形态，EMT相关marker，以及肿瘤特性的检测。结果与对照组相比，共培养之后的MCF7细胞发生上皮间质转化。与haMSC共培养能明显促进乳腺癌细胞的侵袭和迁移，但对细胞周期和增殖能力没有明确的影响。结论人脂肪来源的间充质干细胞可诱导乳腺癌细胞上皮间质转化。

关键词: 间充质干细胞，乳腺癌，上皮间质转化，肿瘤转移

Abstract: Objective To study the effect of human adipose tissue derived MSCs (haMSC) in breast tumor micro-environment on breast cancer cells. Methods Breast cancer cell lines (MCF7) were cultured alone or indirectly co-cultured with MSCs using transwell system. The co-cultured MCF7 cells were collected for analysis of the changes in morphology, EMT related marker and tumor characteristics. Results Following indirect co-culture with MSCs, breast cancer cells morphology and EMT specific markers altered in response to MSCs. The invasive capacity and migration ability of MCF7 cells post co-cultured with MSCs were significantly enhanced, while no changes were observed in cell cycle and proliferation. Conclusion Human adipose tissue derived MSCs can stimulate epithelial to mesenchymal transition of breast cancer cells.

Key words: Mesenchymal stem cell, Breast cancer, Epithelial to mesenchymal transition, Tumor metastasis

徐琦璘赵春华王椋. 人脂肪来源的间充质干细胞诱导人乳腺癌MCF7细胞上皮间质转化[J]. 基础医学与临床, 2012, 32(6): 623-627.

Qi-Lin XU Chun-hua ZHAO Liang WANG. Mesenchymal stem cells stimulate epithelial to mesenchymal transition in breast cancer cell line MCF7[J]. Basic & Clinical Medicine, 2012, 32(6): 623-627.