川芎嗪对大鼠急性脊髓损伤模型NF-kB及I-kBα表达的影响

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (4): 407-412.

川芎嗪对大鼠急性脊髓损伤模型NF-kB及I-kBα表达的影响

肖志满¹,胡建中²,吕红斌³,卓祥龙³

1. 福建省晋江市医院
2. 湖南省中南大学湘雅医院
3. 湖南省中南大学湘雅医学院

收稿日期:2011-03-03 修回日期:2011-10-08 出版日期:2012-04-05 发布日期:2012-03-21
通讯作者: 胡建中 E-mail:hjz3734@vip.sina.com
基金资助:
湖南省自然科学基金（09JJ3047），湖南省卫生厅科研基金（B2007037）

The effect of tetramethylpyrazine on the expression of NF-kB and I-kBα after acute spinal cord injury in the rat model

Received:2011-03-03 Revised:2011-10-08 Online:2012-04-05 Published:2012-03-21

摘要/Abstract

摘要： 【摘要】目的：观察川芎嗪对大鼠急性脊髓损伤模型损伤段NF-kB及I-kBα表达的影响。方法：SD大鼠110只，随机分正常对照组、单纯脊髓损伤组和川芎嗪处理组。采用改良ALLEN氏打击法建立大鼠急性脊髓损伤模型。采用改良Rivlin斜板实验、BBB(Basso，Beattie，Bresnahan)及CBS（Combine Behavior Score）评分对大鼠脊髓功能进行行为学评分。在建模后1h、3h、6h、12h、1d、3d、5d、7d、14d和21d获取损伤段脊髓标本，HE染色观察其组织病理变化；免疫组织化学染色检测NF-kB及I-kBα表达。结果：随着时间推移，术后斜板临界度数和BBB评分值均逐渐升高，CBS评分值逐渐降低，且术后7、14和21d川芎嗪处理组斜板临界角度数均较单纯脊髓损伤组高（P＜0.05），术后5、7、14和21d实验组BBB评分值均较单纯脊髓损伤组高（P＜0.05），而术后5、7、14和21d，川芎嗪处理组CBS评分值均较对照组低（P＜0.05）。术后1、3、5和7d，川芎嗪处理组NF-kB阳性细胞表达数较单纯脊髓损伤组低（P＜0.05）,I-kBα阳性细胞表达数较单纯脊髓损伤组高（P＜0.05）。结论：川芎嗪能够促进大鼠急性继发性损伤后脊髓组织中I-kBα的表达，抑制NF-kB的表达,从而起到促进脊髓功能恢复的作用。

关键词: 【关键词】：脊髓损伤, 川芎嗪, 核因子-kappaB, 抑制性卡巴蛋白

Abstract: Abstract: Objective To study the effect of Teramethylpyrazine (TMP) on expression of NF-kB and I-kBα in acute spinal cord injury(ASCI) in rats. Methods Allen’S weight drop method was used to establish acute spinal cord injury(SCI) rat model at T10 section and was conducted in 110 adult Sprague Dawley rats that were subsequently divided into Sham operation group (group S， n=10)；Control group (group C，n=50)；Tramethylpyrazine group (group T，n=50). The function of spinal cord was evaluated with animal behavioral scores by measuring modified Rivilin loxotic plate test and counting BBB and CBS score at 1、3、5、7、14and 21d postoperatively．The injured spinal cord tissue samples were harvested at 1h、3h、6h、12h、1d、3d、5d、7d、14d and 21d postoperatively(n=5 for each time point) for the preparation of continuous histological sections that were analyzed the expression of NF-kB and I-kBα by immunohistochemistry method．Results T group had significantly higher degrees than C group at 7、14 and 21 days in modified Rivlin loxotic plate test after SCI (p＜0.05)；T group had significantly higher scores in BBB score than C group at 5、7、14 and 21 days after SCI respectively(p＜0.05).C group had significantly higher scores in CBS score than T group at 5、7、14 and 21 days after SCI respectively(p＜0.05). The number of NF-kB positive cells were significantly lower in T group than C group at 1、3、5 and 7 days after SCI(p＜0.05); The number of I-kBα positive cells were significantly lower in C group than T group at 1、3、5 and 7 days after SCI(p＜0.05). Conclusion The TMP can promote the repair of injured spinal cord tissue through increasing the I-kBα expression and decreasing the NF-kB expressions in cells after ASCI.

Key words: Key words: spinal cord injury, tetramethylpyrazine, NF-kB, I-kBα

肖志满胡建中吕红斌卓祥龙. 川芎嗪对大鼠急性脊髓损伤模型NF-kB及I-kBα表达的影响[J]. 基础医学与临床, 2012, 32(4): 407-412.