骨髓间充质干细胞对衰老大鼠肠损伤修复作用的研究

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (9): 1026-1031.

骨髓间充质干细胞对衰老大鼠肠损伤修复作用的研究

王志红,郭坤元,邓兰,曲佳,杨理婷

南方医科大学珠江医院

收稿日期:2010-06-25 修回日期:2010-11-27 出版日期:2011-09-05 发布日期:2011-09-05
通讯作者: 郭坤元 E-mail:gzyuan@pub.Guanzhou.cn.com

Bone marrow mesenchymal stem cells transplantation for the reduction of intestine injury in aging rat

Received:2010-06-25 Revised:2010-11-27 Online:2011-09-05 Published:2011-09-05

摘要/Abstract

摘要： 目的探讨骨髓间充质干细胞（MSCs）在D-半乳糖制备的衰老大鼠小肠损伤中的作用。方法SD大鼠30只，随机均分为3组：对照组、衰老模型组和MSCs防治组。给衰老模型组大鼠每日皮下注射D-半乳糖400mg/kg，连续4个月。MSCs防治组在衰老模型制备成功后，给予尾静脉输注3×106 个MSCs。分别采用硫代巴比妥酸和黄嘌呤氧化法检测小肠组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性；观察3组大鼠小肠组织结构的差异，并用表达绿色荧光蛋白（GFP）的慢病毒载体标记MSCs，以确定MSCs的植入情况。结果 GFP标记的MSCs移植给大鼠后，能向小肠组织迁移并存活。与衰老模型组相比，MSCs防治组SOD 的活性明显升高，MDA含量明显降低，差异有显著性意义，分别为：（133.7±3.6），（105.1±4.3）U/ml，P <0.01；（5.9±0.1），（6.9±0.1）(nmol/ml)，P <0.01。模型组大鼠肠道粘膜损伤严重，而MSCs防治组大鼠的小肠损伤有明显修复。结论骨髓间充质干细胞能够一定程度上减轻D-半乳糖致衰老大鼠的小肠损伤。

关键词: 间充质干细胞, 衰老, 小肠

Abstract: Objective To observe the effect of bone-marrow mesenchymal stem cells on intestine in the aging rats induced by D-galactose. Methods Healthy SD rats were divided into three groups at random: normal control group, aging model group and MSCs group. The aging model group were injected with D-galactose for 4 months to establish the aging rats. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in intestine were measured wtih hydroxylamine and chromatometry respectively. The pathological morphological changes of intestine were observed. MSCs were transfected by lentiviral vectors expressing green fluorescent protein (GFP) to construct GFP-MSCs, and the migration of MSCs in intestine was examined. Results MSCs marked by GFP could migrate into the intestine. Compared with the aging model group，the activity of SOD was significantly lower，the content of MDA increased significantly in MSCs group, and there were significant differences:（133.7±3.6）, （105.1±4.3）U/ml, P <0.01; （5.9±0.1）, （6.9±0.1）(nmol/ml), P <0.01. The intestinal mucosa injury is serious in aging model group rats; and the intestine pathological injury had obvious repaired in MSCs group, compared with aging model group. Conclusion These results support the idea that transplantation of mesenchymal stem showed a protective effect on intestine damage induced by D-galactose.

Key words: mesenchymal stem cells, aging, intestine

中图分类号:

王志红郭坤元邓兰曲佳杨理婷. 骨髓间充质干细胞对衰老大鼠肠损伤修复作用的研究 [J]. 基础医学与临床, 2011, 31(9): 1026-1031.

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