TRAIL及其受体在自身免疫性炎性肌病大鼠肺组织中的表达

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (5): 540-545.

TRAIL及其受体在自身免疫性炎性肌病大鼠肺组织中的表达

赵华¹,王国春²

1. 北京协和医学院
2. 中日友好医院

收稿日期:2011-01-04 修回日期:2011-03-14 出版日期:2011-05-05 发布日期:2011-05-06
通讯作者: 王国春 E-mail:guochunwang@hotmail.com

Expression of TRAIL and TRAIL receptors in interstitial lung disease of experimental autoimmune myositis

Hua ZHAO¹,Guo-chun WANG²

1. Peking Union Medical College
2. China-Japan Friendship Hospital

Received:2011-01-04 Revised:2011-03-14 Online:2011-05-05 Published:2011-05-06
Contact: Guo-chun WANG E-mail:guochunwang@hotmail.com

摘要/Abstract

摘要： 目的探讨自身免疫性炎性肌病肺间质病变的病因及发病机制。方法用异种动物骨骼肌匀浆免疫诱导建立自身免疫性炎性肌病大鼠模型，HE染色和Masson染色观察肌肉和肺组织病理改变，免疫组织化学染色结合计算机图像分析方法检测TRAIL及其受体在病变肺组织中的表达。结果 45例自身免疫性炎性肌病大鼠中有16只出现不同程度的肺间质病变 (P<0.05)；病变肺组织中TRAIL表达由对照组的2035±657升高至8934±741(P<0.05)，DCR1表达由对照组的6985±497降低至1996±401 (P<0.05)，局部炎性细胞浸润高表达DCR2；肺泡炎性反应程度与肺组织中CD8+T淋巴细胞浸润相关（P<0.01），肺纤维化程度与肺组织中TRAIL表达升高相关（P<0.05）。结论本研究首次证实自身免疫性炎性肌病大鼠模型出现与特发性炎性肌病患者相似肺间质病变，且与局部异常活跃的免疫炎性反应有关，TRAIL及其受体可能参与其中。

关键词: 自身免疫性炎性疾病, 肺间质病变, 组织病理, TRAIL及其受体

Abstract: Objective To investigate the pathologies and pathogenesis of interstitial lung disease (ILD) in experimental autoimmune myositis (EAM) models. Methods Established EAM mode by injecting the homogenate of rabbit skeletal muscle with Freund's complete adjuvant. The histopathological features of the muscle and lung from the EAM model were investigated by staining with hematoxylin and eosin and Masson's trichrome. The expressions of TRAIL and TRAIL receptors (TRAIL-R1, TRAIL-R2, TRAIL-R3, and TRAIL-R4) in lung tissues were measured by immunohistochemical method and computer image analysis. Result 16 of 45 rats with autoimmune inflammatory myopathies were found varying degrees of interstitial lung disease (P <0.05); TRAIL expression was increased in lung tissue (39265±27595) and that of DCR1 was decreased (37377±14126) (P <0.05); Inflammatory cells infiltrated locally were found with high expression of DCR2; Alveolitis was related with CD8 + T lymphocytes (P <0.001) and pulmonary fibrosis was related with TRAIL expression in lung tissues (P <0.05). Conclusion The study firstly demonstrates that the features of ILD shown in EAMs were related with the local immune inflammatory response. The changes in the expression of TRAIL and its receptors between EAMs and controls suggest TRAIL-system probably play a role in the pathogenesis and the pathology of IIM with ILD.

Key words: EAM, ILD, Histopathology, TRAIL and TRAIL receptors

中图分类号:

R593

赵华王国春. TRAIL及其受体在自身免疫性炎性肌病大鼠肺组织中的表达[J]. 基础医学与临床, 2011, 31(5): 540-545.

Hua ZHAO Guo-chun WANG. Expression of TRAIL and TRAIL receptors in interstitial lung disease of experimental autoimmune myositis[J]. Basic & Clinical Medicine, 2011, 31(5): 540-545.

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