Erastin诱导人非小细胞肺癌细胞系A549凋亡

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (9): 1313-1317.

Erastin诱导人非小细胞肺癌细胞系A549凋亡

姜学东¹, 李文雅^2*

1.中国人民武装警察部队辽宁省总队医院胸外科,辽宁沈阳 110034;
2.中国医科大学附属第一医院胸外科,辽宁沈阳 110001

收稿日期:2020-12-25 修回日期:2021-06-08 出版日期:2021-09-05 发布日期:2021-09-02
通讯作者: *41578203@qq.com

Erastin induces cell apoptosis in human non-small cell lung cancer cell line A549

JIANG Xue-dong¹, LI Wen-ya^2*

1. Department of Thoracic Surgery, Liaoning Provincial Corps Hospital, Chinese People's Armed Police Forces, Shenyang 110034;
2. Department of Thoracic Surgery, the First Affiliated Hospital, China Medical University, Shenyang 110001, China

Received:2020-12-25 Revised:2021-06-08 Online:2021-09-05 Published:2021-09-02
Contact: *41578203@qq.com

摘要/Abstract

摘要： 目的探讨爱拉斯汀(erastin)对非小细胞肺癌细胞系A549凋亡的诱导作用及机制。方法不同浓度的erastin处理A549细胞,加入/不加入活性氧集团(ROS)清除剂N -乙酰基-L-半胱氨酸(NAC),加入/不加入凋亡抑制剂(Z-VAD-FMK);试剂盒检测细胞铁含量;CCK-8法和流式细胞测量术检测细胞损伤和凋亡等。结果 Erastin引起A549细胞铁离子含量升高和细胞损伤,诱导细胞凋亡(P＜0.05),该作用可被凋亡caspase抑制剂Z-VAD-FMK抑制;Erastin引起过氧化物(ROS)增加从而诱导细胞凋亡(P＜0.05),该作用可被ROS清除剂NAC减弱(P＜0.05)。结论 Erastin通过诱导非小细胞肺癌细胞系A549内ROS产生,引起细胞凋亡,该发现为非小细胞肺癌的诊疗策略提供了参考。

关键词: 爱拉斯汀, 非小细胞肺癌, 凋亡

Abstract: Objective To explore whether erastin induces apoptosis and to investigate the molecular mechanism in A549 cells. Methods A549 cells were treated with erastin at different concentrations, with or without ROS scavenging agent N-acetyl-L-cysteine (NAC) and with or without apoptosis inhibitor (Z-VAD-FMK). Then iron content was measured with Iron Assay Kit, cell injure and apoptosis with CCK-8 and flow cytometry in A549 cells. Results Erastin induced the increase of iron content and followed by the injury of A549 cells. Erastin further induced apoptosis of A549 cells (P＜0.05), which was inhibited by apoptosis inhibitor Z-VAD-FMK. Erastin induced an increase in peroxides (ROS) then followed by apoptosis(P＜0.05), which was inhibited by the ROS scavenging agent NAC(P＜0.05). Conclusions Erastin has an inhibitory effect on NSCLC A549 by inducing intracellular ROS production, which provides evidences for the pathogenesis, diagnosis and treatment of NSCLC.

Key words: erastin, non-small cell lung cancer, apoptosis

中图分类号:

R394-33

姜学东, 李文雅. Erastin诱导人非小细胞肺癌细胞系A549凋亡[J]. 基础医学与临床, 2021, 41(9): 1313-1317.

JIANG Xue-dong, LI Wen-ya. Erastin induces cell apoptosis in human non-small cell lung cancer cell line A549[J]. Basic & Clinical Medicine, 2021, 41(9): 1313-1317.

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