miR-26a调控PTEN基因降低小鼠慢性淋巴白血病细胞的耐药性

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (6): 871-875.

miR-26a调控PTEN基因降低小鼠慢性淋巴白血病细胞的耐药性

毛洪宾^1*, 何明²

1.开封市人民医院临床药学科,河南开封 475000;
2.河南大学第一附属医院临床药学科,河南开封 475001

收稿日期:2020-04-27 修回日期:2020-10-13 出版日期:2021-06-05 发布日期:2021-05-31
通讯作者: ^*pei2611@sohu.com

Effect of miR-26a on drug resistance of chronic lymphocytic leukemia cells by regulating PTEN gene

MAO Hong-bin^1*, HE Ming²

1. Department of Clinical Pharmacy, Kaifeng People's Hospital, Kaifeng 475000;
2. Department of Clinical Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng 475001, China

Received:2020-04-27 Revised:2020-10-13 Online:2021-06-05 Published:2021-05-31
Contact: ^*pei2611@sohu.com

摘要/Abstract

摘要： 目的观察微小RNA-26a(miR-26a)通过第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)对慢性淋巴细胞白血病(CLL)细胞耐药性的调控作用。方法实验分为4组:对照组(L1210细胞),耐药组(L1210/DDP细胞)、NC组(转染空载质粒+L1210/DDP细胞)和抑制剂组(转染miR-26a-inhibitor质粒+L1210/DDP细胞)。RT-qPCR检测miR-26a和PTEN mRNA表达;双荧光素酶实验检测miR-26a对PTEN的靶向性;MTT法检测细胞对顺铂(DDP)耐药性;流式细胞仪检测细胞凋亡率;Wetern blot检测细胞中PTEN、磷脂酰肌醇3激酶(PI3K)、丝氨酸-苏氨酸蛋白激酶(AKT)蛋白表达。结果 miR-26a能够抑制细胞中PTEN表达(P＜0.05)。miR-26a可靶向作用于PTEN。抑制miR-26a表达可降低细胞对DDP的半数抑制浓度(IC₅₀)值,提高细胞凋亡率,上调PI3K蛋白表达及p-AKT/AKT(P＜0.05)。结论抑制miR-26a表达可通过上调PTEN表达降低细胞耐药作用。

关键词: 慢性淋巴细胞白血病, 耐药性, 微小RNA-26a, 第10号染色体缺失的磷酸酶及张力蛋白同源基因

Abstract: Objective To observe the regulation of microRNA-26a (miR-26a) on the drug resistance of chronic lymphocyte leukemia (CLL) cells through the phosphatase and tensin homolog deleted on chromosome ten (PTEN). Methods The experiment was divided into 4 groups: control group (L1210 cells), resistance group (L1210/DDP cells), NC group (transfected with empty plasmid+L1210/DDP cells), inhibitor group (transfected with miR-26a-inhibitor plasmid+L1210/DDP and inhibitor group (transfected with miR-26a-inhibitor plasmid+L1210/DDP cells). miR-26a and PTEN mRNA expression were detected by RT-qPCR. Dual luciferase experiment was used to detect the targeting of miR-26a to PTEN. MTT method was used to detect cell resistance to cisplatin (DDP). Flow cytometry was used to detect cell apoptosis rates. Western blot was used to detect of PTEN, phosphatidyl inositol-3-kinase (PI3K), serine-threonine protein kinase (AKT) protein expressions in cells. Results miR-26a inhibited the expression of PTEN in cells (P＜0.05). miR-26a targeted PTEN. Inhibiting the expression of miR-26a reduced the IC₅₀ value of cells to DDP, increased the rates of apoptosis, and up-regulated the expression of PI3K protein and p-AKT/AKT (P＜0.05). Conclusions Inhibiting the expression of miR-26a can reduces the effect of cell resistance through up-regulating the expression of PTEN.

Key words: chronic lymphocyte leukemia, drug resistance, microRNA-26a, phosphatase and tensin homolog deleted on chromosome ten

中图分类号:

R733.72

毛洪宾, 何明. miR-26a调控PTEN基因降低小鼠慢性淋巴白血病细胞的耐药性[J]. 基础医学与临床, 2021, 41(6): 871-875.

MAO Hong-bin, HE Ming. Effect of miR-26a on drug resistance of chronic lymphocytic leukemia cells by regulating PTEN gene[J]. Basic & Clinical Medicine, 2021, 41(6): 871-875.

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