原花青素抑制Aβ25-35诱导的人神经母细胞瘤细胞系SH-SY5Y分泌Aβ1-42

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (5): 704-708.

原花青素抑制Aβ_25-35诱导的人神经母细胞瘤细胞系SH-SY5Y分泌Aβ_1-42

李琪^1,2, 张小强^1,2*, 陶一凡^1,2, 朱孟雯^1,2, 崔丹丹^1,2, 孙腾腾^1,2

东南大学 1.公共卫生学院; 2.环境医学工程教育部重点实验室, 江苏南京 210009

收稿日期:2020-12-09 修回日期:2021-03-20 出版日期:2021-05-05 发布日期:2021-05-06
通讯作者: ^*zhangxq7843@126.com
基金资助:
中央高校基本科研业务费专项资金资助和江苏省普通高校研究生科研创新计划资助项目(SJZZ16_0034)

Proanthocyanidins inhibit the secretion of Aβ_1-42 induced by Aβ_25-35 in human neuroblastoma cell line SH-SY5Y

LI Qi^1,2, ZHANG Xiao-qiang^1,2*, TAO Yi-fan^1,2, ZHU Meng-wen^1,2, CUI Dan-dan^1,2, SUN Teng-teng^1,2

1. School of Public Health; 2. Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University,Nanjing 210009,China

Received:2020-12-09 Revised:2021-03-20 Online:2021-05-05 Published:2021-05-06
Contact: ^*zhangxq7843@126.com

摘要/Abstract

摘要： 目的研究原花青素(PC)对β-淀粉样肽(Aβ_25-35)诱导的人神经母细胞瘤细胞系(SH-SY5Y)淀粉样肽产生的影响及可能的作用机制。方法以Aβ_25-35体外处理SH-SY5Y细胞诱导细胞损伤模型,应用PC和(或)Wnt/β-catenin信号通路的内源性抑制剂分泌型蛋白Dickkopf-1(Dkk1)干预。设立对照组、Aβ_25-35组、不同浓度PC+Aβ_25-35组、Dkk1组和Dkk1+PC+Aβ_25-35组。MTT法检测细胞活力;Western blot检测β-catenin、GSK-3β和p-GSK-3β蛋白表达水平;ELISA检测Aβ_1-42分泌水平。结果与对照组相比,Aβ_25-35可使细胞活力降低(P＜0.05),可降低细胞中β-catenin蛋白和p-GSK-3β蛋白表达水平(P＜0.05),增加细胞中Aβ_1-42分泌(P＜0.05)。PC干预可改善Aβ_25-35导致的细胞活力降低(P＜0.05),增加细胞中β-catenin蛋白和p-GSK-3β蛋白表达水平(P＜0.05),减少Aβ_1-42分泌(P＜0.05)。应用Dkk1后,PC提高细胞中β-catenin蛋白和p-GSK-3β蛋白表达水平及降低Aβ_1-42分泌水平的能力下降(P＜0.05)。结论 PC可抑制Aβ_25-35诱导的SH-SY5Y细胞Aβ_1-42生成,该作用可能与Wnt/β-catenin信号通路有关。

关键词: 原花青素, Aβ_1-42, Wnt/β-catenin

Abstract: Objective To investigate the effect of proanthocyanidins(PC) induced by amyloid-beta peptide(Aβ_25-35) in human neuroblastoma cell line SH-SY5Y and its possible mechanism. Methods SH-SY5Y cells were exposed to Aβ_25-35 to establish a cell injury model in vitro. PC and(or) secretory protein Dickkopf-1 (Dkk1), the endogenic inhibitor of Wnt/β-catenin signaling pathway were used for intervention. The cells were divided into control group, Aβ_25-35 group, PC(different concentrations)+Aβ_25-35 groups, Dkk1 group, Dkk1+ PC+Aβ_25-35 group.The cell viability was evaluated by MTT assay. The expressions of β-catenin, GSK-3β, p-GSK-3β were measured by Western blot. The secreted Aβ_1-42 was detected by ELISA. Results Compared with the control group, the cell viability, the expression of β-catenin and p-GSK-3β were reduced in Aβ_25-35 treated group(P＜0.05), while the secreted Aβ_1-42 was increased (P＜0.05). PC improved the cell viability, increased the expression of β-catenin and p-GSK-3β, and reduced the secreted Aβ_1-42 in SH-SY5Y cells induced by Aβ_25-35 (P＜0.05). After using Dkk1,the ability of PC was decreased on the enhancement of β-catenin expression, the improvement of p-GSK-3β expression, and the reduction of the secreted Aβ_1-42 (P＜0.05). Conclusions PC may decrease Aβ_1-42 secretion in SH-SY5Y cells induced by Aβ_25-35 through Wnt/β-catenin signaling pathway.

Key words: proanthocyanidins, Aβ_1-42, Wnt/β-catenin

中图分类号:

R749.16

李琪, 张小强, 陶一凡, 朱孟雯, 崔丹丹, 孙腾腾. 原花青素抑制Aβ_25-35诱导的人神经母细胞瘤细胞系SH-SY5Y分泌Aβ_1-42[J]. 基础医学与临床, 2021, 41(5): 704-708.

LI Qi, ZHANG Xiao-qiang, TAO Yi-fan, ZHU Meng-wen, CUI Dan-dan, SUN Teng-teng. Proanthocyanidins inhibit the secretion of Aβ_1-42 induced by Aβ_25-35 in human neuroblastoma cell line SH-SY5Y[J]. Basic & Clinical Medicine, 2021, 41(5): 704-708.

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