酸枣仁黄酮减轻全氟辛烷磺酸诱导小鼠肝脏氧化损伤

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (2): 184-188.

酸枣仁黄酮减轻全氟辛烷磺酸诱导小鼠肝脏氧化损伤

肖凤琴¹, 张红印¹, 韩荣欣¹, 张荣榕¹, 严铭铭^1*, 张世阳², 贾秀秀²

1.长春中医药大学吉林省中药保健食品科技创新中心,吉林长春 130117;
2.上海纤连生物技术有限公司,上海 200120

收稿日期:2019-11-29 修回日期:2020-12-18 出版日期:2021-02-05 发布日期:2021-01-19
通讯作者: ^*yanmm595@yahoo.com.cn
基金资助:
吉林省长春市科技创新重大专项(17YJ007);吉林省科技发展项目科技创新(工程技术中心)-科技创新中心建设(20180623041TC)

Ziziphi Spinosae Semen Flavonoids alleviates liver oxidative damage induced by perfluorooctane sulfonate in mice

XIAO Feng-qin¹, ZHANG Hong-yin¹, HAN Rong-xin¹, ZHANG Rong-rong¹, YAN Ming-ming^1*, ZHANG Shi-yang², JIA Xiu-xiu²

1. Changchun University of Chinese Medicine, Chinese Medicine Health Food Science and Technologyinnovation Center of Jilin Province, Changchun 130117;
2. Shanghai Fiberlink Biotechnology Co. Ltd., Shanghai 200120, China

Received:2019-11-29 Revised:2020-12-18 Online:2021-02-05 Published:2021-01-19
Contact: ^*yanmm595@yahoo.com.cn

摘要/Abstract

摘要： 目的通过全氟辛烷磺酸(PFOS)诱导小鼠肝脏氧化损伤模型来研究酸枣仁黄酮(ZSSF)对其的保护作用。方法将小鼠随机分为空白组、造模组、维生素C组和ZSSF高、中、低剂量组,每组10只。灌胃给药同时用PFOS(10 mg/kg)造模,连续给药30 d。末次给药6 h后,取小鼠眼球血,取肝脏加入9倍体积的0.9% 氯化钠溶液于匀浆器中制成10 % 的组织匀浆液,采用ELLSA法对血清中的丙转氨酶(ALT)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷草转氨酶(AST)、丙二醛(MDA)、总抗氧化能力(T-AOC)和超氧化物歧化酶 (SOD)等因子进行定量分析;同时应用RT-qPCR技术对肝组织中Nrf2、HO-1 mRNA的表达进行检测。结果造模组与空白组相比,ALT、AST、MDA含量升高,SOD、GSH-Px、CAT和T-AOC含量降低(P＜0.01,P＜0.05);给药干预组与模型组比较,减少了AST、ALT和MDA的含量,提高了SOD、CAT、GSH-Px、T-AOC含量以及肝组织中Nrf2、HO-1 mRNA的表达(P＜0.01,P＜0.05)。结论 ZSSF具有改善PFOS 诱导的小鼠氧化性肝损伤的作用,其可能的作用机制是通过调节Nrf2/HO-1途径增加体内抗氧化酶的活性,从而抑制过氧化脂质的合成。

关键词: 酸枣仁黄酮, NF-E2-related factor 2, heme oxygenase-1, 全氟辛烷磺酸, 机制研究

Abstract: Objective To study the protective effect of Ziziphi Spinosae Semen Flavonoids (ZSSF) on liver oxidative damage induced by perfluorooctane sulfonate (PFOS) in mice. Methods Mice were divided into blank control group, model group, positive control group (vitamin C group) and high, medium and low dose ZSSF group by random number table method, with 10 mice in each group. At the same time, PFOS (10 mg/kg) was molded and administered continuously for 30 days.After 6 h after the last delivery, eyeball blood in mice, the liver to join 9 times the volume of 0.9% sodium chloride solution in homogenate is made from 10% of the tissue of slurry, according to the kit instruction alanine transaminase (ALT) in serum and aspirate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (gsh-px) and catalase (CAT) and total antioxidant capacity (T -AOC) level; The expression of Nrf2 and HO-1 mrna in liver tissues was detected, and real-time fluorescent quantitative PCR(RT-qPCR) was applied. Results Compared with the control group, the contents of alanine aminotransferase, aspartate aminotransferase, and malondialdehyde increased in the model group, while the contents of superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity decreased(P＜ 0.01, P＜0.05); Compared with the model group, the drug intervention group reduced the content of AST, ALT and MDA, and increased the content of SOD, CAT, GSH-Px, T-AOC, Nrf2 and HO-1 mRNA in liver tissue(P＜0.01, P＜0.05). Conclusions Ziziphi Spinosae Semen Flavonoids can improve the oxidative liver injury induced by PFOS in mice, which may be caused by increasing the activity of antioxidant enzymes in vivo and reducing the generation of lipid peroxides by regulating the Nrf2/HO-1 pathway, so as to improve the antioxidant capacity of the body.

Key words: Ziziphi Spinosae Semen Flavonoids, NF-E2-related factor 2, heme oxygenase-1, perfluorooctane sulfonate, mechanism research

中图分类号:

R285

肖凤琴, 张红印, 韩荣欣, 张荣榕, 严铭铭, 张世阳, 贾秀秀. 酸枣仁黄酮减轻全氟辛烷磺酸诱导小鼠肝脏氧化损伤[J]. 基础医学与临床, 2021, 41(2): 184-188.

XIAO Feng-qin, ZHANG Hong-yin, HAN Rong-xin, ZHANG Rong-rong, YAN Ming-ming, ZHANG Shi-yang, JIA Xiu-xiu. Ziziphi Spinosae Semen Flavonoids alleviates liver oxidative damage induced by perfluorooctane sulfonate in mice[J]. Basic & Clinical Medicine, 2021, 41(2): 184-188.

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