大鼠脾切除对移植脂肪间充质干细胞的肝脏归巢效率及肝硬化的影响

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (10): 1428-1433.

大鼠脾切除对移植脂肪间充质干细胞的肝脏归巢效率及肝硬化的影响

唐卫平¹, 李翀¹, 陈国栋¹, 赵磊^2*

南华大学附属第一医院 1.肝胆胰外科; 2.胃肠外科,湖南衡阳 421001

收稿日期:2020-10-23 修回日期:2021-04-15 发布日期:2021-09-29
通讯作者: *akahoshi2012@163.com
基金资助:
国家自然科学基金青年科学项目(81900488)

Effects of splenectomy on liver homing and cirrhosis therapy after transplantation with adipose tissue derived mesenchymal stem cells in rats

TANG Wei-ping¹, LI Chong¹, CHEN Guo-dong¹, ZHAO Lei^2*

1. Department of Hepatobiliary and Pancreatic Surgery;
2. Department of Gastrointestinal Surgery, the First Affiliated Hospital,University of South China, Hengyang 421001, China

Received:2020-10-23 Revised:2021-04-15 Published:2021-09-29
Contact: *akahoshi2012@163.com

摘要/Abstract

摘要： 目的探讨脾切除对移植脂肪间充质干细胞(ADSCs)的肝脏归巢及肝硬化的影响。方法肝硬化大鼠建模后随机分为4组:假手术组(sham)、假手术+ADSCs移植组(ADSCs)、脾切除组(Sp)和脾切除+ADSCs移植组(Sp+ADSCs)。假手术或脾切术后7 d经尾静脉注射ADSCs,7 d后检测肝功能、肝脏病理改变和ADSCs在肝内的归巢效率。结果分离的大鼠ADSCs CD29呈阳性且具备脂肪和软骨分化能力。ADSCs组肝纤维化面积较假手术组明显缩小(P＜0.05)。与ADSCs组相比,Sp+ADSCs组的肝纤维化面积进一步缩小(P＜0.05)。ADSCs组肝组织collagen Ⅰ mRNA表达较sham组下调(P＜0.05),且Sp+ADSCs组collagen Ⅰ mRNA表达较ADSCs组进一步降低。Sp+ADSCs组ALT水平为(213±89)U/L,低于ADSCs组(280±56)U/L(P＜0.05)。Sp+ADSCs组肝组织中荧光信号强度高于ADSCs组(P＜0.05)。结论脾切除提高ADSCs在肝内的归巢效率并增强ADSCs对肝硬化的疗效。

关键词: 脾切除, 脂肪间充质干细胞, 归巢, 肝硬化

Abstract: Objective To explore the influence of splenectomy on liver homing and treatment of cirrhosis after adipose tissue derived mesenchymal stem cells (ADSCs) infusion. Methods The rats liver cirrhosis model was established and randomly divided into four groups, sham operation group (sham), sham operation + ADSCs infusion group (ADSCs), splenectomy group (Sp), splenectomy + ADSCs infusion group (Sp+ADSCs). The cell infusion was performed one week after sham or splenectomy. Then, liver function, pathological and homing changes were detected one week later. Results The isolated ADSCs were CD29 positive and underwent adipogenic and chondrogenic differentiation, the hepatic fibrosis area in ADSCs group was significantly smaller than that sham operation group (P＜0.05), and the fibrotic area in Sp+ADSCs group reduced as compared with that ADSCs group(P＜0.05). The expression of collagen Ⅰ mRNA in ADSCs group was significantly decreased as compared with that in sham group(P＜0.05), and mRNA coding collagen Ⅰ in Sp+ADSCs group was further decreased as compared with that in ADSCs group. As compared with ADSCs group[ALT: (280±56)U/L], Sp+ADSCs group [ALT: (213±89)U/L] had a significant difference in inhibiting liver injury (P＜0.05); Ex vitro fluorescence imaging showed that the fluorescence signal intensity in liver tissue of the Sp+ADSCs was significantly higher than that in ADSCs group (P＜0.05). Conclusions Splenectomy can significantly improve the live homing of ADSCs and improve their therapeutic effect on liver cirrhosis.

Key words: splenectomy, adipose-derived mesenchymal stem cells, homing, liver cirrhosis

中图分类号:

R575

唐卫平, 李翀, 陈国栋, 赵磊. 大鼠脾切除对移植脂肪间充质干细胞的肝脏归巢效率及肝硬化的影响[J]. 基础医学与临床, 2021, 41(10): 1428-1433.

TANG Wei-ping, LI Chong, CHEN Guo-dong, ZHAO Lei. Effects of splenectomy on liver homing and cirrhosis therapy after transplantation with adipose tissue derived mesenchymal stem cells in rats[J]. Basic & Clinical Medicine, 2021, 41(10): 1428-1433.

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