基础医学与临床 ›› 2021, Vol. 41 ›› Issue (1): 72-76.

• 研究论文 • 上一篇    下一篇

α-硫辛酸对自身免疫性甲状腺炎小鼠炎性反应的抑制作用

玛依努·玉苏甫1,2,衣巴地古丽?库吐鲁克1,2,哈尼克孜?阿不都艾尼1,2   

  1. 1. 新疆维吾尔自治区人民医院
    2. 新疆维吾尔自治区人民医院
  • 收稿日期:2019-11-22 修回日期:2020-09-07 出版日期:2021-01-05 发布日期:2020-12-30
  • 通讯作者: 玛依努·玉苏甫 E-mail:691978893@qq.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金资助项目

Inhibition of inflammatory response by α-lipoic acid in autoimmune thyroiditis mice

  • Received:2019-11-22 Revised:2020-09-07 Online:2021-01-05 Published:2020-12-30

摘要: 目的 探讨α-硫辛酸抑制免疫性甲状腺炎小鼠(AIT)炎性反应及对淋巴细胞亚群的影响。方法 将80只NOD.H-2h 4雌鼠随机分为对照组,给予无菌双蒸水灌胃;AIT 组给予50 mg/L碘化钠无菌双蒸水灌胃;低及高剂量α-硫辛酸干预组每天腹腔注射0.1和0.4 g/L α-硫辛酸。每组20只,均持续干预8周。干预8周后,用HE染色观察淋巴细胞浸润,采用流式细胞仪检测Th17、Treg、CD4+、CD8+ T细胞及CD4+/CD8+水平,采用Western-blot法测定P38MAPK、NF-κB蛋白水平。结果 AIT组小鼠甲状腺炎性反应发生率高达85%。AIT+低/高剂量α-硫辛酸组小鼠甲状腺炎发生率、甲状腺炎性反应程度评分以及Th17 细胞比例显著低于AIT组(P<0.05),Treg、CD4+、CD8+以及CD4 + /CD8 +细胞比例显著高于AIT 组(P<0. 05)。AIT+低/高剂量α-硫辛酸组的蛋白相对表达水平较AIT组明显降低(P<0.05)。结论 α-硫辛酸可以降低自身免疫性甲状腺小鼠炎性反应因子表达,改善淋巴细胞亚群的比例,从而抑制炎性反应和改善AIT症状。

Abstract: OBJECTIVE: To investigate the inhibitory effect of α-lipoic acid on immune thyroiditis mice (AIT) and its effect on lymphocyte subsets. METHODS: Eighty NOD.H-2h 4 female rats were randomly divided into a control group and given double distilled water for gavage; the AIT group was given 50 mg/L sodium iodide sterile double distilled water for gavage ; Low- and high-dose α-lipoic acid intervention group was injected intraperitoneally with 0.1 and 0.4 g/L α-lipoic acid daily. Twenty animals in each group continued to intervene for 8 weeks. After 8 weeks of intervention, HE staining was used to observe lymphocyte infiltration, flow cytometry was used to detect Th17, Treg, CD4+, CD8+ T cells and CD4+/CD8+ levels, and Western-blot method was used to determine P38MAPK and NF-κB protein levels. RESULTS: The incidence of thyroid inflammation of mice in the AIT group was as high as 85%. The incidence of thyroiditis, the degree of thyroid inflammation, and the proportion of Th17 cells in the AIT + low / high-dose alpha-lipoic acid group were significantly lower than those in the AIT group (P <0.05), and the proportion of Treg, CD4+, CD8+, and CD4+/CD8+ cells were significant Higher than that in the AIT group (P <0.05). The AIT + low / high-dose α-lipoic acid group had significantly lower relative protein expression levels than those in the AIT group (P <0.05). Conclusion: α-lipoic acid can reduce the expression of inflammatory cytokines in autoimmune thyroid mice, improve the proportion of lymphocyte subsets, thereby suppressing the inflammatory response and improving the symptoms of AIT.